Response adaptive mechanisms of three mangrove (Avicennia marina, Aegiceras corniculatum, and Bruguiera gymnorrhiza) plants to waterlogging stress revealed by transcriptome analysis
Response adaptive mechanisms of three mangrove (Avicennia marina, Aegiceras corniculatum, and Bruguiera gymnorrhiza) plants to waterlogging stress revealed by transcriptome analysis
复制标题
转录组分析揭示三种红树林（白骨壤、桐花树和木榄）对涝害胁迫的响应适应机制
DOI：
10.3389/fmars.2022.929649
复制
发表时间：
2022-12
影响因子：
3.7
通讯作者：
Cui-Ci Sun
中科院分区：
文献类型：
--
作者：
Bo-Yu Su;You-Shao Wang;Cui-Ci Sun
红树植物分布在潮间带的不同海拔高度，经历不同的淹水时期，从生理结构到代谢机制都具有不同的耐涝适应性。结合物种分布，采用转录组测序的方法，探讨了白骨壤、桐花树和木本植物在淹水胁迫下的分子响应机制的种间差异。结果表明，胁迫后滨海对虾差异表达基因数(Deg)最高，Ae次之。角藻和木犀草。研究发现，在这三种植物中，差异表达折叠(8倍以上)基因的功能可分为四类：结构调节、运输、生物合成和保护。此外，还发现滨海拟青霉在代谢过程中具有较强的调节能力，能够在淹水条件下保证能量供应并保持活跃的生物合成。此外，海棠在乙烯合成途径中被激活，以促进通风组织的形成，避免根组织的缺氧。不同于A.marina和B.ginorRha，Ae.角藻下调了XET、SAMS和ACCO基因的表达，这些基因参与了细胞壁的调节或乙烯的形成，这可能表明了不同的适应机制。乙醇脱氢酶(ADH)和乳酸脱氢酶(LDH)是从A.marina、Ae.和木兰根(B.GymnorRha)。分别命名为AmADH、AmLDH、AcADH、AcLDH、BgADH和BgLDH。QRT-PCR检测证实LDHs和ADHS参与了红树植物对渍水胁迫的响应，并存在种间差异。AcADH和AcLDH的表达最为突出。结合转录组分析，认为Ae.角藻更依赖于AcADH和AcLDH的表达，这可能弥补了细胞壁调节的不足，而滨海角藻则更依赖于细胞结构的调节。
Distributed in different elevations of the intertidal zone, mangrove plants suffer different periods of flooding, and with varied adaptability to waterlogging from the physiological structure to the metabolic mechanism. Associated with species distribution, transcriptome sequencing was performed to explore the interspecific differences of molecular response mechanisms among Avicennia marina, Aegiceras corniculatum, and Bruguiera gymnorrhiza under waterlogging stress. Results showed that the counts of differentially expressed genes (DEGs) in A. Marina were the highest after stress, followed by Ae. corniculatum and B. gymnorrhiza. It was found that the functions of genes with high differential expression folds (more than eight folds) in the three plants could be classified into four categories: structural regulation, transport, biosynthesis, and protection. It was also found that A. Marina has strong regulation ability in the metabolic process, which can guarantee energy supply and maintain active biosynthesis under waterlogging conditions. In addition, A. Marina was activated in the ethylene synthesis pathway to promote aerenchyma formation and to avoid root tissue hypoxia. Being different from A. Marina and B. gymnorrhiza, Ae. corniculatum down-regulated the XET, SAMS, and ACCO genes, which were involved in the cell wall regulation or ethylene formation that might indicate a different adaptive mechanism. Alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH) were cloned from A. Marina, Ae. corniculatum, and B. gymnorrhiza. The cloned genes were named as AmADH, AmLDH, AcADH, AcLDH, BgADH, and BgLDH, respectively. qRT-PCR detection verified that LDHs and ADHs were involved in the response of mangrove plants to waterlogging stress, and interspecific difference was observed. The expressions of AcADH and AcLDH were the most prominent. Combined with transcriptome, it was considered that Ae. corniculatum was more dependent on the expression of AcADH and AcLDH that might compensate the weakness of cell wall regulation, whereas A. Marina was more dependent on the regulation of cell structure reversely.
登录
查看更多内容
影响因子：
4.2
作者：
HOVENDEN, MJ;ALLAWAY, WG
通讯作者：
ALLAWAY, WG
影响因子：
4.8
作者：
You-Shao Wang;J. Gu
通讯作者：
You-Shao Wang;J. Gu
影响因子：
1.4
作者：
B. Vartapetian
通讯作者：
B. Vartapetian
影响因子：
4.3
作者：
R. Peretto;F. Favaron;V. Bettini;G. Lorenzo;S. Marini;P. Alghisi;F. Cervone;P. Bonfante
通讯作者：
R. Peretto;F. Favaron;V. Bettini;G. Lorenzo;S. Marini;P. Alghisi;F. Cervone;P. Bonfante
影响因子：
5.7
作者：
Y. Ye;N. Tam;Y. Wong;C. Lu
通讯作者：
Y. Ye;N. Tam;Y. Wong;C. Lu