Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity.
Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity.
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作者：
Liu J;Reeves C;Jacques T;McEvoy A;Miserocchi A;Thompson P;Sisodiya S;Thom M
巢蛋白在未成熟的神经上皮细胞和祖细胞类型中表达，并在对急性脑损伤（包括癫痫发作）作出反应的增殖神经胶质细胞中瞬时上调。在36例颞叶癫痫患者（年龄范围8-60岁）的颞叶（Tlobe）标本中，我们研究了26例（年龄范围12孕周至76岁）尸检对照组织中足、海马体、海马旁回、杏仁核、颞叶皮质和极中巢蛋白表达细胞（NEC）的数量、分布和形态。使用MCM 2在选定区域（包括公认的小生境）中评价NEC的增殖分数。用神经元（DCX、mushashi、βIII微管蛋白、NeuN）和神经胶质（GFAP、GFAP δ、谷氨酰胺合成酶、水通道蛋白4、EAAT 1）标记物在切片中或培养后探索它们的分化。结果与临床参数相关。观察到NEC的分布和形态的定型模式，使人想起发育中大脑的模式，癫痫患者的密度高于成人对照组（p <0.001）。结果包括室周白色物质中的MCM 2阳性放射状胶质样细胞和海马伞和沟中的成排NEC。在癫痫病例中，巢蛋白细胞占海马增殖分数的29%;在培养物中，20%共表达βIII微管蛋白，而GFAP为28%。手术年龄、记忆缺陷和巢蛋白数量之间存在显著相关性。具有持续增殖能力的Tlobe NEC可能代表发育迁移流的痕迹和与海马癫痫发生、Tlobe病理学和共病（包括记忆力下降）潜在相关的驻留反应性细胞群。
Nestin is expressed in immature neuroepithelial and progenitor cell types and transiently upregulated in proliferative neuroglial cells responding to acute brain injury, including following seizures. In 36 temporal lobe (TLobe) specimens from patients with TLobe epilepsy (age range 8–60 years) we studied the number, distribution and morphology of nestin‐expressing cells (NEC) in the pes, hippocampus body, parahippocampal gyrus, amygdala, temporal cortex and pole compared with post mortem control tissues from 26 cases (age range 12 gestational weeks to 76 years). The proliferative fraction of NEC was evaluated in selected regions, including recognized niches, using MCM2. Their differentiation was explored with neuronal (DCX, mushashi, βIII tubulin, NeuN) and glial (GFAP, GFAPdelta, glutamine synthetase, aquaporin4, EAAT1) markers, both in sections or following culture. Findings were correlated with clinical parameters. A stereotypical pattern in the distribution and morphologies of NEC was observed, reminiscent of patterns in the developing brain, with increased densities in epilepsy than adult controls (p < .001). Findings included MCM2‐positive radial glial‐like cells in the periventricular white matter and rows of NEC in the hippocampal fimbria and sulcus. Nestin cells represented 29% of the hippocampal proliferative fraction in epilepsy cases; 20% co‐expressed βIII tubulin in culture compared with 28% with GFAP. Significant correlations were noted between age at surgery, memory deficits and nestin populations. TLobe NEC with ongoing proliferative capacity likely represent vestiges of developmental migratory streams and resident reactive cell populations of potential relevance to hippocampal epileptogenesis, TLobe pathology, and co‐morbidities, including memory decline.
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影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
Gage, FH
影响因子：
5.6
作者：
ElAli A;Thériault P;Rivest S
通讯作者：
Rivest S
影响因子：
5.3
作者：
Burda JE;Bernstein AM;Sofroniew MV
通讯作者：
Sofroniew MV
影响因子：
3.7
作者：
Kamphuis W;Mamber C;Moeton M;Kooijman L;Sluijs JA;Jansen AH;Verveer M;de Groot LR;Smith VD;Rangarajan S;Rodríguez JJ;Orre M;Hol EM
通讯作者：
Hol EM
影响因子：
14.5
作者：
Helmstaedter, C.;Elger, C. E.
通讯作者：
Elger, C. E.