Sweep imaging with Fourier transform as a tool with MRI for evaluating the effect of teriparatide on cortical bone formation in an ovariectomized rat model.
Sweep imaging with Fourier transform as a tool with MRI for evaluating the effect of teriparatide on cortical bone formation in an ovariectomized rat model.
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DOI：
10.1186/s12891-021-04970-7
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发表时间：
2022-01-03
影响因子：
2.3
通讯作者：
Takahashi K
中科院分区：
文献类型：
--
作者：
Sotozono Y;Ikoma K;Kido M;Onishi O;Minami M;Wada H;Yamada S;Matsuda KI;Tanaka M;Takahashi K
Teriparlitazone（TPTD）是一种治疗骨质疏松症的药物，可促进骨形成并改善骨质量。然而，TPTD对皮质骨的影响还不清楚。扫描成像与傅里叶变换（SWIFT）已被报道为一个有用的工具，用于评估结合水的皮质骨，但它尚未被用来研究TPTD对皮质骨的影响。本研究旨在使用SWIFT评价TPTD对皮质骨形成的影响。在卵巢切除术后饲养15周龄雌性Sprague-Dawley大鼠（n = 36）以建立绝经后骨质疏松症模型。将患者分为TPTD组和非TPTD组。在开始TPTD给药后4、12和24周对大鼠实施安乐死。胫骨进行了评估，使用磁共振成像（MRI）和骨组织形态计量学。在MRI中，进行质子密度加权成像（PDWI）和SWIFT成像。计算每种方法的信噪比（SNR）。然后使用MRI评估的相同区域通过骨组织形态计量学计算骨形成率。使用Mann-Whitney U检验比较测量值，P值< 0.05被认为是显著的。在任何时间点，两组之间的PDWI-SNR均无显著差异（4、12和24周时分别为P = 0.589、0.394和0.394）。首先，在开始治疗后4周，TPTD组的SWIFT-SNR显著高于非TPTD组，但在12周和24周时无显著差异（4、12和24周时分别为P = 0.009、0.937和0.818）。在所有时间点，TPTD组通过组织形态计量学评估的骨形成率均显著高于非TPTD组（P < 0.05，所有周）。特别是在第4周，TPTD组的骨形成率显著高于非TPTD组（P = 0.028，1.98 ± 0.33 vs. 0.09 ± 0.05 μm3/μm2/天）。SWIFT可检测到结合水信号增加，反映了TPTD对皮质骨的影响。SWIFT检测到的信号反映了皮质骨形成率的显著增加。
Teriparatide (TPTD) is a drug for osteoporosis that promotes bone formation and improves bone quality. However, the effects of TPTD on cortical bone are not well understood. Sweep imaging with Fourier transform (SWIFT) has been reported as a useful tool for evaluating bound water of cortical bone, but it has yet to be used to investigate the effects of TPTD on cortical bone. This study aimed to evaluate the consequences of the effect of TPTD on cortical bone formation using SWIFT. Twelve-week-old female Sprague-Dawley rats (n = 36) were reared after ovariectomy to create a postmenopausal osteoporosis model. They were divided into two groups: the TPTD and non-TPTD groups. Rats were euthanized at 4, 12, and 24 weeks after initiating TPTD treatment. Tibial bones were evaluated using magnetic resonance imaging (MRI) and bone histomorphometry. In MRI, proton density-weighted imaging (PDWI) and SWIFT imaging were performed. The signal-to-noise ratio (SNR) was calculated for each method. The same area evaluated by MRI was then used to calculate the bone formation rate by bone histomorphometry. Measurements were compared using the Mann-Whitney U-test, and a P-value of < 0.05 was considered significant. PDWI-SNR was not significantly different between the two groups at any time point (P = 0.589, 0.394, and 0.394 at 4, 12, and 24 weeks, respectively). Contrarily, SWIFT-SNR was significantly higher in the TPTD group than in the non-TPTD group at 4 weeks after initiating treatment, but it was not significantly different at 12 and 24 weeks (P = 0.009, 0.937, and 0.818 at 4, 12, and 24 weeks, respectively). The bone formation rate assessed by histomorphometry was significantly higher in the TPTD group than in the non-TPTD group at all timepoints (P < 0.05, all weeks). In particular, at 4 weeks, the bone formation rate was markedly higher in the TPTD group than in the non-TPTD group (P = 0.028, 1.98 ± 0.33 vs. 0.09 ± 0.05 μm3/μm2/day). SWIFT could detect increased signals of bound water, reflecting the effect of TPTD on the cortical bone. The signal detected by SWIFT reflects a marked increase in the cortical bone formation rate.
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DOI：
10.1007/bf02058659
发表时间：
1969-01-01
期刊：
CALCIFIED TISSUE RESEARCH
影响因子：
--
作者：
ROCKOFF, SD;SWEET, E;BLEUSTEIN, J
通讯作者：
BLEUSTEIN, J
影响因子：
4
作者：
Sugimoto, T.;Nakamura, T.;Nakamura, Y.;Isogai, Y.;Shiraki, M.
通讯作者：
Shiraki, M.
对医疗统计信息的自由使用的易于使用的软件“ EZR”的调查。
影响因子：
4.8
作者：
Kanda Y
通讯作者：
Kanda Y
影响因子：
4.1
作者：
Zebaze, Roger;Takao-Kawabata, Ryoko;Seeman, Ego
通讯作者：
Seeman, Ego
影响因子：
5.8
作者：
Chavassieux, Pascale;Portero-Muzy, Nathalie;Chapurlat, Roland
通讯作者：
Chapurlat, Roland