In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
In vitro study of the inflammatory cells response to biodegradable Mg-based alloy extract.
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可生物降解镁基合金提取物炎症细胞反应的体外研究
DOI：
10.1371/journal.pone.0193276
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Chen T
中科院分区：
文献类型：
--
作者：
Jin L;Wu J;Yuan G;Chen T
可生物降解的镁基合金在骨固定装置和血管支架方面显示出巨大的潜力。异物反应(FBR)作为植入物的生物材料，是一个重要的研究课题，其中炎症细胞起着关键作用。在这里，我们使用两种炎性细胞系，即THP-1细胞和THP-1巨噬细胞，以评价镁-钕-锌-锆合金(简称JDBM)提取物对细胞活力、死亡模式、细胞周期、吞噬、分化、迁移和炎症反应的影响。结果表明，高浓度提取液可引起细胞功能的完全损伤和坏死。中浓度提取液可见细胞发生凋亡，细胞功能部分受损。20%浓度的提取物与巨噬细胞共培养可上调巨噬细胞肿瘤坏死因子-α的表达，而相同浓度的提取物可下调巨噬细胞的肿瘤坏死因子-DNA的表达。有趣的是，当巨噬细胞在不依赖内毒素的中高浓度提取物中培养时，肿瘤坏死因子-α的产生减少。JDBM提取物中的镁离子也对细胞活力产生负面影响，这是影响细胞功能的一个潜在因素。我们的结果为镁合金提取物对免疫细胞表型的影响及其可能的机制提供了新的信息，在临床应用之前应予以考虑。
Biodegradable Mg-based alloys have shown great potential as bone fixation devices or vascular stents. As implant biomaterials, the foreign body reaction (FBR) is an important issue to be studied, where the inflammatory cells play a key role. Here, we used two inflammatory cell lines i.e. THP-1 cells and THP-1 macrophages, to evaluate the effect of Mg–Nd–Zn–Zr alloy (denoted as JDBM) extracts on cell viability, death modes, cell cycle, phagocytosis, differentiation, migration and inflammatory response. The results showed that high-concentration extract induced necrosis and complete damage of cell function. For middle-concentration extract, cell apoptosis and partially impaired cell function were observed. TNF-α expression of macrophages was up-regulated by co-culture with extract in 20% concentration, but was down-regulated in the same concentration in the presence of LPS stimulation. Interestingly, the production of TNF-α decreased when macrophages were cultured in middle and high concentration extracts independent of LPS. Cell viability was also negatively affected by magnesium ions in JDBM extracts, which was a potential factor affecting cell function. Our results provide new information about the impact of Mg alloy extracts on phenotype of immune cells and the potential mechanism, which should be taken into account prior to clinical applications.
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影响因子：
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