Cytocompatibility and early inflammatory response of human endothelial cells in direct culture with Mg-Zn-Sr alloys.
Cytocompatibility and early inflammatory response of human endothelial cells in direct culture with Mg-Zn-Sr alloys.
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DOI：
10.1016/j.actbio.2016.10.020
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发表时间：
2017-01-15
影响因子：
9.7
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Cipriano AF;Sallee A;Tayoba M;Cortez Alcaraz MC;Lin A;Guan RG;Zhao ZY;Liu H
结晶镁-锌（Zn）-锶（Sr）三元合金由人体中天然存在的元素组成，并为各种生物医学应用提供有吸引力的机械和生物降解性能。本研究的第一个目的是研究四种Mg-4 Zn-xSr合金（x = 0.15，0.5，1.0，1.5 wt%;分别命名为Mg 41 A，B，C，D）在体外与人脐静脉内皮细胞（HUVEC）直接培养中的降解和细胞相容性。第二个目的是调查，第一次，在培养的HUVECs的早期炎症反应所示的诱导血管细胞粘附分子-1（VCAM-1）。结果表明，Zn/Sr比为1.5的β相Al_2O_3 - 41合金的24 h体外降解明显快于Zn/Sr比为1.5的Al_2O_3 - 41合金。此外，粘附密度的HUVEC在直接培养，但不直接接触的Al 2 O3 - 41合金长达24小时没有受到不利影响的合金的降解。重要的是，补充有高达27.6 mM Mg 2+离子的培养基和有意调节至碱性pH 9的培养基都不会对HUVEC应答产生任何可检测的不良影响。相比之下，显著更高的，但非细胞毒性的，Zn 2+离子浓度从降解的Al 2 Or 41 D合金可能是最初较高的VCAM-1表达培养的HUVEC的原因。最后，分析HUVEC-Ehr 41界面显示几乎完全没有直接在样品表面上的细胞粘附，这很可能是由高局部碱度、表面形貌变化和/或表面组成引起的。在这项研究中使用的直接培养方法被提出作为一个有价值的工具，在体外研究含锌镁基生物材料的设计方面，以工程解决方案，以解决目前的缺点，镁合金血管装置的应用。
Crystalline Mg-Zinc (Zn)-Strontium (Sr) ternary alloys consist of elements naturally present in the human body and provide attractive mechanical and biodegradable properties for a variety of biomedical applications. The first objective of this study was to investigate the degradation and cytocompatibility of four Mg-4Zn-xSr alloys (x = 0.15, 0.5, 1.0, 1.5 wt%; designated as ZSr41A, B, C, and D respectively) in the direct culture with human umbilical vein endothelial cells (HUVEC) in vitro. The second objective was to investigate, for the first time, the early-stage inflammatory response in cultured HUVECs as indicated by the induction of vascular cellular adhesion molecule-1 (VCAM-1). The results showed that the 24-h in vitro degradation of the ZSr41 alloys containing a β-phase with a Zn/Sr at% ratio ~1.5 was significantly faster than the ZSr41 alloys with Zn/Sr at% ~1. Additionally, the adhesion density of HUVECs in the direct culture but not in direct contact with the ZSr41 alloys for up to 24 h was not adversely affected by the degradation of the alloys. Importantly, neither culture media supplemented with up to 27.6 mM Mg2+ ions nor media intentionally adjusted up to alkaline pH 9 induced any detectable adverse effects on HUVEC responses. In contrast, the significantly higher, yet non-cytotoxic, Zn2+ ion concentration from the degradation of ZSr41D alloy was likely the cause for the initially higher VCAM-1 expression on cultured HUVECs. Lastly, analysis of the HUVEC-ZSr41 interface showed near-complete absence of cell adhesion directly on the sample surface, most likely caused by either a high local alkalinity, change in surface topography, and/or surface composition. The direct culture method used in this study was proposed as a valuable tool for studying the design aspects of Zn-containing Mg-based biomaterials in vitro, in order to engineer solutions to address current shortcomings of Mg alloys for vascular device applications.
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影响因子：
3.9
作者：
Bondarenko A;Hewicker-Trautwein M;Erdmann N;Angrisani N;Reifenrath J;Meyer-Lindenberg A
通讯作者：
Meyer-Lindenberg A
影响因子：
11.4
作者：
Cortese-Krott, Miriam M.;Kulakov, Larissa;Oplaender, Christian;Kolb-Bachofen, Victoria;Kroencke, Klaus-D.;Suschek, Christoph V.
通讯作者：
Suschek, Christoph V.
影响因子：
4.6
作者：
Cha, Pil-Ryung;Han, Hyung-Seop;Yang, Gui-Fu;Kim, Yu-Chan;Hong, Ki-Ha;Lee, Seung-Cheol;Jung, Jae-Young;Ahn, Jae-Pyeong;Kim, Young-Yul;Cho, Sung-Youn;Byun, Ji Young;Lee, Kang-Sik;Yang, Seok-Jo;Seok, Hyun-Kwang
通讯作者：
Seok, Hyun-Kwang
影响因子：
2.9
作者：
Feser, K.;Kietzmann, M.;Bach, F. W.
通讯作者：
Bach, F. W.
人工生物液成分对潜在骨科Mg-Ca、AZ31、AZ91合金生物腐蚀的影响
影响因子：
4
作者：
Gu, X. N.;Zheng, Y. F.;Chen, L. J.
通讯作者：
Chen, L. J.