[Gd@C(82)(OH)(22)](n) nanoparticles induce dendritic cell maturation and activate Th1 immune responses.
[Gd@C(82)(OH)(22)](n) nanoparticles induce dendritic cell maturation and activate Th1 immune responses.
复制标题
DOI：
10.1021/nn901478z
复制
发表时间：
2010-02-23
期刊：
影响因子：
17.1
通讯作者：
Zhang N
中科院分区：
文献类型：
--
作者：
Yang D;Zhao Y;Guo H;Li Y;Tewary P;Xing G;Hou W;Oppenheim JJ;Zhang N
树突状细胞在宿主免疫防御中起着关键作用，如清除外来病原体和抑制肿瘤发生。在本文中，我们报道了[Gd@C82(OH)22]n可以通过刺激DC产生包括IL-12p70在内的细胞因子，上调DC共刺激（CD80、CD83和CD86）和MHC （HLA-A、B、C和HLA-DR）分子，并将DC从ccl5应答型转换为ccl19应答型，从而诱导树突状细胞的表型成熟。我们发现[Gd@C82(OH)22]n可以诱导树突状细胞在功能上成熟，正如它们激活同种异体T细胞的能力所示。在[Gd@C82(OH)22]n存在的情况下，用卵清蛋白免疫的小鼠表现出增强的卵清蛋白特异性th1极化免疫反应，主要是IFNγ、IL-1β和IL-2的产生增加。[Gd@C82(OH)22]n纳米颗粒是树突状细胞和Th1免疫反应的有效激活剂。这些新发现也为先前报道的[Gd@C82(OH)22]n纳米颗粒的有效抗癌活性提供了合理的理解。
Dendritic cells play a pivotal role in host immune defense, such as elimination of foreign pathogen and inhibition of tumorigenesis. In this paper, we report that [Gd@C82(OH)22]n could induce phenotypic maturation of dendritic cells by stimulating DC production of cytokines including IL-12p70, upregulating DC costimulatory (CD80, CD83, and CD86) and MHC (HLA-A,B,C and HLA-DR) molecules, and switching DCs from a CCL5-responsive to a CCL19-responsive phenotype. We found that [Gd@C82(OH)22]n can induce dendritic cells to become functionally mature as illustrated by their capacity to activate allogeneic T cells. Mice immunized with ovalbumin in the presence of [Gd@C82(OH)22]n exhibit enhanced ovalbumin-specific Th1-polarized immune response as evidenced by the predominantly increased production of IFNγ, IL-1β, and IL-2. The [Gd@C82(OH)22]n nanoparticle is a potent activator of dendritic cells and Th1 immune responses. These new findings also provide a rational understanding of the potent anticancer activities of [Gd@C82(OH)22]n nanoparticles reported previously.
登录
查看更多内容
影响因子：
4.1
作者：
Dugan, LL;Lovett, EG;O'Malley, KL
通讯作者：
O'Malley, KL
影响因子：
2.7
作者：
Mashino, T;Okuda, K;Mochizuki, M
通讯作者：
Mochizuki, M
影响因子：
15
作者：
Akasaka, Takeshi;Kono, Takayoshi;Nagase, Shigeru
通讯作者：
Nagase, Shigeru
影响因子：
14
作者：
Liu, Ying;Jiao, Fang;Chen, Chunying
通讯作者：
Chen, Chunying
影响因子：
4.7
作者：
Mikawa, M;Kato, H;Shinohara, H
通讯作者：
Shinohara, H