Hawthorn Leaf Flavonoids Protect against Diabetes-Induced Cardiomyopathy in Rats via PKC-α Signaling Pathway.
Hawthorn Leaf Flavonoids Protect against Diabetes-Induced Cardiomyopathy in Rats via PKC-α Signaling Pathway.
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DOI：
10.1155/2017/2071952
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lv W
中科院分区：
文献类型：
--
作者：
Min Q;Bai Y;Zhang Y;Yu W;Zhang M;Liu D;Diao T;Lv W
DCM已成为糖尿病患者死亡的主要原因之一。本研究旨在探讨山楂叶总黄酮（HLF）对糖尿病大鼠心脏损伤的保护作用及其机制。采用高脂饲料喂养8周后，腹腔注射链脲佐菌素（STZ, 40 mg/kg）建立实验性糖尿病模型。糖尿病大鼠接受不同剂量的HLF（50、100和200）治疗16周。光镜下观察心肌细胞形态学变化；测定各组抗氧化指标、TNF-α浓度及PKC-α mRNA、PKC-α、NF-κB蛋白表达。stz诱导的糖尿病可引起血糖升高、心脏损伤、氧化应激和炎症反应，并伴有体重抑制。相反，给药HLF可改善糖尿病大鼠的体重和血糖，减轻心肌结构异常。此外，HLF降低MDA水平，提高SOD活性，抑制TNF-α表达，下调糖尿病诱导的PKC-α mRNA、PKC-α、NF-κB表达。HLF对大鼠糖尿病性心肌病有保护作用。其机制可能涉及通过PKC-α信号通路失活来减少氧化应激和炎症。
DCM has become one of the main reasons of death in diabetic patients. In this study, we aimed to explore the hawthorn leaf flavonoids (HLF) protective effect against diabetes-induced cardiac injury and the underlying mechanisms in experimental rats. Experimental diabetic model was induced by intraperitoneal injection of streptozotocin (STZ, 40 mg/kg) in rats after feeding with high-fat diet for 8 weeks. The diabetic rats received a 16-week treatment of different doses of HLF (50, 100, and 200). The morphological changes of myocardial cells were observed by light microscope; the concentration of antioxidant indicator and TNF-α and the expression of PKC-α mRNA, PKC-α, and NF-κB proteins were assessed as well. STZ-induced diabetes mellitus prompted blood glucose, cardiac injury, oxidative stress, and inflammation, accompanied with suppressed body weight. On the contrary, HLF administration improved body weight and blood glucose and attenuated myocardial structural abnormalities in diabetic rats. In addition, HLF decreased MDA level and enhanced SOD activities, inhibited TNF-α expression, and downregulated PKC-α mRNA, PKC-α, and NF-κB which were induced by diabetes. HLF has a protective effect against diabetic cardiomyopathy in rats. The mechanism may be involved in reducing oxidative stress and inflammation via inactivation of the PKC-α signaling pathway.
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影响因子：
5.1
作者：
Guariguata, L.;Whiting, D. R.;Shaw, J. E.
通讯作者：
Shaw, J. E.
影响因子：
--
作者：
Ayala A;Muñoz MF;Argüelles S
通讯作者：
Argüelles S
DOI：
10.1152/ajpheart.00583.2005
发表时间：
2006-01-01
影响因子：
4.8
作者：
Greer, JJM;Ware, DP;Lefer, DJ
通讯作者：
Lefer, DJ
影响因子：
4.3
作者：
Joshi, Mandip;Kotha, Sainath R.;Maulik, Nilanjana
通讯作者：
Maulik, Nilanjana
影响因子：
2.8
作者：
Haffner, SM
通讯作者：
Haffner, SM