A WUSCHEL-like homeobox gene, OsWOX3B responses to NUDA/GL-1 locus in rice.
A WUSCHEL-like homeobox gene, OsWOX3B responses to NUDA/GL-1 locus in rice.
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水稻中类似 WUSCHEL 的同源盒基因 OsWOX3B 对 NUDA/GL-1 位点的反应
DOI：
10.1186/1939-8433-5-30
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Teng S
中科院分区：
文献类型：
--
作者：
Zhang H;Wu K;Wang Y;Peng Y;Hu F;Wen L;Han B;Qian Q;Teng S
水稻品种大多数为短柔毛。然而，毛状体的存在是水稻生产中不期望的特征，因为毛状体可引起大气污染。使用无毛水稻品种代表了解决这个问题的方法。云南裸稻是一种无毛水稻品种，约占云南水稻种质资源的20%，可为培育无毛水稻品种提供重要资源。结果云南裸稻的“裸稻”表型受GL-1位点的一对隐性等位基因控制。利用云南裸花油菜品种HMK与短柔毛油菜品种TN 1杂交获得的F2群体的1,192个裸花油菜个体构建了高分辨率的遗传图谱和物理图谱。NUDA/GL-1基因定位在28.5kb的区域内，包含6个基于日本晴基因组序列的注释基因。通过比较不同短柔毛和无毛品种的基因序列和表达模式，确定LOC_Os05g02730、aWUSCHEL样同源框基因（OsWOX 3B）为候选基因。这一假说已被RNA干扰（RNAi）和转基因互补所证实。结论NUDA/GL-1基因座编码OsWOX 3B基因。
BackgroundMost of the rice varieties are pubescent. However, the presence of trichomes is an undesirable characteristic in rice production because trichomes can cause atmospheric pollution. The use of glabrous rice varieties represents a solution to this problem. Yunnan Nuda Rice, a glabrous cultivar that constitutes approximately 20% of rice germplasms in Yunnan can provide important recourse for breeding of glabrous rice varieties.ResultsThe “Nuda” phenotype in Yunnan Nuda Rice was found to be controlled by a single recessive allelic gene within the well-characterizedGL-1locus. A high-resolution genetic and physical map was constructed using 1,192 Nuda individuals from the F2population that was delivered from the cross between the Yunnan Nuda variety HMK and the pubescent TN1 variety. TheNUDA/GL-1gene was mapped to a 28.5 kb region containing six annotated genes based on the Nipponbare genomic sequence. By comparing the sequences and expression patterns of different pubescent and glabrous varieties,LOC_Os05g02730, aWUSCHEL-like homeobox gene (OsWOX3B) was identified as the candidate gene. This hypothesis was confirmed by RNA interference (RNAi) and transgenic complementation. Trichome deficiency in RNAi lines was associated with increased efficiency of grain packaging but did not affect the main agronomic traits.ConclusionNUDA/GL-1locus encodesOsWOX3Bgene.
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影响因子：
10.7
作者：
Nardmann, Judith;Zimmermann, Roman;Werr, Wolfgang
通讯作者：
Werr, Wolfgang
影响因子：
7.4
作者：
Dai, Mingqiu;Hu, Yongfeng;Zhou, Dao-Xiu
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影响因子：
2.3
作者：
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MAITI, RK
影响因子：
4.6
作者：
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通讯作者：
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影响因子：
7.2
作者：
Kamiya, N;Nagasaki, H;Matsuoka, M
通讯作者：
Matsuoka, M