Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin.
Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin.
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DOI：
10.1016/j.scr.2013.11.010
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发表时间：
2014-03
影响因子：
1.2
通讯作者：
Robey, Pamela G.
中科院分区：
文献类型：
--
作者：
Mallon, Barbara S.;Hamilton, Rebecca S.;Kozhich, Olga A.;Johnson, Kory R.;Fann, Yang C.;Rao, Mahendra S.;Robey, Pamela G.
Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and generated isogenic iPSC lines by reprogramming. The gene expression and methylation profile of three lines were compared to the parental line and intermediate NPC population. We found no gene probe with expression that differed significantly between hESC and iPSC samples under undifferentiated or differentiated conditions. Analysis of the global methylation pattern also showed no significant difference between the two PSC populations. Both undifferentiated populations were distinctly different from the intermediate NPC population in both gene expression and methylation profiles. One point to note is that H1 is a male line and so extrapolation to female lines should be cautioned. However, these data confirm our previous findings that there are no significant differences between hESCs and hiPSCs at the gene expression or methylation level.
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影响因子：
3.7
作者：
Teichroeb JH;Betts DH;Vaziri H
通讯作者：
Vaziri H
影响因子：
3.7
作者：
Marchetto MC;Yeo GW;Kainohana O;Marsala M;Gage FH;Muotri AR
通讯作者：
Muotri AR
影响因子：
46.9
作者：
Deng, Jie;Shoemaker, Robert;Xie, Bin;Gore, Athurva;LeProust, Emily M.;Antosiewicz-Bourget, Jessica;Egli, Dieter;Maherali, Nimet;Park, In-Hyun;Yu, Junying;Daley, George Q.;Eggan, Kevin;Hochedlinger, Konrad;Thomson, James;Wang, Wei;Gao, Yuan;Zhang, Kun
通讯作者：
Zhang, Kun
影响因子：
64.5
作者：
Soldner F;Hockemeyer D;Beard C;Gao Q;Bell GW;Cook EG;Hargus G;Blak A;Cooper O;Mitalipova M;Isacson O;Jaenisch R
通讯作者：
Jaenisch R
影响因子：
7
作者：
Lo, HS;Wang, ZN;Lee, MP
通讯作者：
Lee, MP