Lactobacilli and bifidobacteria promote immune homeostasis by modulating innate immune responses to human rotavirus in neonatal gnotobiotic pigs.
Lactobacilli and bifidobacteria promote immune homeostasis by modulating innate immune responses to human rotavirus in neonatal gnotobiotic pigs.
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DOI：
10.1371/journal.pone.0076962
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Vlasova AN;Chattha KS;Kandasamy S;Liu Z;Esseili M;Shao L;Rajashekara G;Saif LJ
在4组无菌（Gn）猪中评估了与鼠李糖乳杆菌GG（LGG）和乳酸双歧杆菌Bb 12（Bb 12）共定植对减毒HRV 3次接种和强毒人轮状病毒（VirHRV）攻击的影响：Pro+Vac（益生菌定植/接种）、Vac（接种）、Pro（益生菌定植，未接种）和对照（未定植，未接种）。在[攻毒后第0天（PCD）]和（PCD 7）-VirHRV攻毒后对猪亚组实施安乐死，以评估腹泻、粪便HRV脱落和树突状细胞/先天免疫应答。攻毒后，Pro+Vac和Vac组完全免于腹泻;对HRV脱落的保护率分别为100%和83%。在VirHRV攻击后，与对照猪相比，Pro中的腹泻和HRV脱落减少。与所有其他组相比，对照组的腹泻评分和病毒脱落显著更高，与攻毒后血清干扰素-α水平显著更高一致。LGG+ Bb 12定植±疫苗促进免疫成熟，如通过在攻毒后肠组织和血液中表达CD 4、SWC 3a、CD 11 R1、MHCII的单核细胞（MNC）和常规树突状细胞的频率增加所反映的。定殖降低了来自接种疫苗的猪（Pro+Vac）在攻击前表达Toll样受体（TLR）2和TLR 4的MNC的频率，并且增加了来自Pro猪在攻击后表达TLR 3的MNC的频率，这表明益生菌可能通过TLR信号传导发挥抗炎（TLR 2和4下调）和抗病毒（通过HRV dsRNA上调TLR 3）作用。单独的益生菌定植（Pro）增加了肠道和全身凋亡MNCs的频率，从而调节免疫高反应性和耐受性。然而，这些频率在肠道和全身组织的挑战后，缓和HRV诱导的细胞凋亡。此外，攻击后，Pro+Vac和Pro组具有显著降低的MNC增殖，表明益生菌控制VirHRV攻击后的过度淋巴增殖反应。我们的结论是，在新生Gn猪疾病模型，选定的益生菌有助于免疫成熟，调节免疫稳态和调节疫苗和毒性HRV的影响，从而缓和HRV腹泻。
The effects of co-colonization with Lactobacillus rhamnosus GG (LGG) and Bifidobacterium lactis Bb12 (Bb12) on 3-dose vaccination with attenuated HRV and challenge with virulent human rotavirus (VirHRV) were assessed in 4 groups of gnotobiotic (Gn) pigs: Pro+Vac (probiotic-colonized/vaccinated), Vac (vaccinated), Pro (probiotic-colonized, non-vaccinated) and Control (non-colonized, non-vaccinated). Subsets of pigs were euthanized pre- [post-challenge day (PCD) 0] and post (PCD7)-VirHRV challenge to assess diarrhea, fecal HRV shedding and dendritic cell/innate immune responses. Post-challenge, Pro+Vac and Vac groups were completely protected from diarrhea; protection rates against HRV shedding were 100% and 83%, respectively. Diarrhea and HRV shedding were reduced in Pro compared to Control pigs following VirHRV challenge. Diarrhea scores and virus shedding were significantly higher in Controls, compared to all other groups, coincident with significantly higher serum interferon-alpha levels post-challenge. LGG+Bb12 colonization ±vaccine promoted immunomaturation as reflected by increased frequencies of CD4, SWC3a, CD11R1, MHCII expressing mononuclear cells (MNCs) and conventional dendritic cells in intestinal tissues and blood post-challenge. Colonization decreased frequencies of toll-like receptors (TLR) 2 and TLR4 expressing MNCs from vaccinated pigs (Pro+Vac) pre-challenge and increased frequencies of TLR3 expressing MNCs from Pro pigs post-challenge, suggesting that probiotics likely exert anti-inflammatory (TLR2 and 4 down-regulation) and antiviral (TLR3 up-regulation by HRV dsRNA) actions via TLR signaling. Probiotic colonization alone (Pro) increased frequencies of intestinal and systemic apoptotic MNCs pre-challenge, thereby regulating immune hyperreactivity and tolerance. However, these frequencies were decreased in intestinal and systemic tissues post-challenge, moderating HRV-induced apoptosis. Additionally, post-challenge, Pro+Vac and Pro groups had significantly decreased MNC proliferation, suggesting that probiotics control excessive lymphoproliferative reactions upon VirHRV challenge. We conclude that in the neonatal Gn pig disease model, selected probiotics contribute to immunomaturation, regulate immune homeostasis and modulate vaccine and virulent HRV effects, thereby moderating HRV diarrhea.
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DOI：
10.1128/aem.12.4.295-300.1964
发表时间：
1964-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
MEYER, RC;BOHL, EH;KOHLER, EM
通讯作者：
KOHLER, EM
DOI：
10.1073/pnas.0910307107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Pagnini, Cristiano;Saeed, Rubina;Cominelli, Fabio
通讯作者：
Cominelli, Fabio
影响因子：
--
作者：
Foye OT;Huang IF;Chiou CC;Walker WA;Shi HN
通讯作者：
Shi HN
影响因子：
5.4
作者：
Azevedo, MSP;Yuan, L;Saif, LJ
通讯作者：
Saif, LJ
影响因子：
5.4
作者：
Boshuizen, JA;Reimerink, JHJ;Einerhand, AWC
通讯作者：
Einerhand, AWC