Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope
Surface elasticity imaging of vascular tissues in a liquid environment by a scanning haptic microscope
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利用扫描触觉显微镜对液体环境中的血管组织进行表面弹性成像
DOI：
10.1007/s10047-010-0503-2
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发表时间：
2010
期刊：
影响因子：
1.3
通讯作者：
Nakayama Y
中科院分区：
文献类型：
--
作者：
Oie T;Suzuki H;Murayama Y;Fukuda T;Omata S;Kanda K;Takamizawa K;Nakayama Y
这项研究的目的是通过改进扫描触觉显微镜(SHM)，获得液体环境中自然动脉在微米级和亚平方毫米级的高分辨率弹性分布图像。扫描触觉显微镜是用于表征自然组织硬度的。以小口径猪动脉(直径约3 mm)的圆周切面(厚度1.0 mm)为样本。测量方法是将探头(直径5μm，空间分辨率小于2μm)浸泡在适当深度的生理盐水中。血管组织通过多层分离，高弹力区主要是弹性蛋白(50.8±13.8kPa)，低弹力区主要是胶原和平滑肌细胞(17.0±99.0kpa)，这与以前在高湿度条件下观察到的情况一样。在液体环境中重复测量弹性超过4小时，几乎没有变化，这使得能够在至少1,200×100μm的大面积范围内保持高精度观察，而通过在空气中脱水收缩的样品，弹性随时间增加，其中整个区域的平均弹性在2小时内大约翻了一番。这个简单、廉价的系统允许在接近自然的液体环境中观察血管组织细胞外基质水平的表面弹性的分布。
The objective of this study was to make an elasticity distribution image of natural arteries in a liquid environment at high resolution at the micrometer level and at a wide area at the sub-square millimeter level by improving the scanning haptic microscope (SHM), developed previously for characterization of the stiffness of natural tissues. The circumferential sections (thickness, 1.0 mm) of small-caliber porcine arteries (approximately 3-mm diameter) were used as a sample. Measurement was performed by soaking a probe (diameter, 5 μm; spatial resolution, less than 2 μm) in saline solution at an appropriate depth. The vascular tissues were segregated by multi-layering a high elasticity region with mainly elastin (50.8 ± 13.8 kPa) and a low one with mainly collagen and smooth muscle cells (17.0 ± 9.0 kPa), as observed previously in high humidity conditions. The elasticity was measured repeatedly with little change for over 4 h in a liquid environment, which enabled observation with maintenance of high precision of a large area of at least 1,200 × 100 μm, whereas the elasticity was increased with time by the dehydration of samples with shrinkage in the air, in which an averaged elasticity in the overall area was approximately doubled within 2 h. This simple, inexpensive system allows observation of the distribution of the surface elasticity at the extracellular matrix level of vascular tissues in a liquid environment close to the natural one.
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影响因子：
1.5
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通讯作者：
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DOI：
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影响因子：
4.6
作者：
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DOI：
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发表时间：
2005
期刊：
Artificial Organs Sep;29(9)
影响因子：
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作者：
Hayashida K;Huang H;Kanda K;Yaku H;Nakayama Y.
通讯作者：
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影响因子：
2.2
作者：
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