Regulation of per and cry genes reveals a central role for the D-box enhancer in light-dependent gene expression.
Regulation of per and cry genes reveals a central role for the D-box enhancer in light-dependent gene expression.
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DOI：
10.1371/journal.pone.0051278
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Foulkes NS
中科院分区：
文献类型：
--
作者：
Mracek P;Santoriello C;Idda ML;Pagano C;Ben-Moshe Z;Gothilf Y;Vallone D;Foulkes NS
光作为一个关键的环境信号，使生物钟与昼夜周期同步。斑马鱼是探索光如何影响脊椎动物生物钟机制的一个有吸引力的模型。大多数鱼类组织和细胞系的直接光照诱导广泛的基因表达，包括DNA修复，应激反应和关键时钟基因。我们以前已经确定了D-和E-盒元件内的斑马鱼per 2基因的启动子，共同指导光诱导的基因表达。然而，E盒和D盒的联合调控是所有光诱导基因表达的普遍特征吗？我们通过研究额外的光诱导基因的调控来解决这个问题。我们的研究结果表明，除了per 2，所有其他基因测试不诱导光阻断从头蛋白质合成。我们发现，一个单一的D盒作为主要的光响应元件内的cry 1a启动子。此外，在抑制蛋白质合成时，D盒介导的基因表达被消除，而E盒赋予光驱动的激活，如在per 2基因中观察到的。考虑到鱼细胞中存在不同的光感受器，我们的结果暗示D-box增强子是光驱动信号的一般会聚点。
Light serves as a key environmental signal for synchronizing the circadian clock with the day night cycle. The zebrafish represents an attractive model for exploring how light influences the vertebrate clock mechanism. Direct illumination of most fish tissues and cell lines induces expression of a broad range of genes including DNA repair, stress response and key clock genes. We have previously identified D- and E-box elements within the promoter of the zebrafish per2 gene that together direct light-induced gene expression. However, is the combined regulation by E- and D-boxes a general feature for all light-induced gene expression? We have tackled this question by examining the regulation of additional light-inducible genes. Our results demonstrate that with the exception of per2, all other genes tested are not induced by light upon blocking of de novo protein synthesis. We reveal that a single D-box serves as the principal light responsive element within the cry1a promoter. Furthermore, upon inhibition of protein synthesis D-box mediated gene expression is abolished while the E-box confers light driven activation as observed in the per2 gene. Given the existence of different photoreceptors in fish cells, our results implicate the D-box enhancer as a general convergence point for light driven signaling.
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影响因子：
9.8
作者：
Cavallari N;Frigato E;Vallone D;Fröhlich N;Lopez-Olmeda JF;Foà A;Berti R;Sánchez-Vázquez FJ;Bertolucci C;Foulkes NS
通讯作者：
Foulkes NS
影响因子：
64.8
作者：
LAMPH, WW;WAMSLEY, P;VERMA, IM
通讯作者：
VERMA, IM
影响因子：
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作者：
Cermakian, N;Pando, MP;Sassone-Corsi, P
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Sassone-Corsi, P
影响因子：
30.8
作者：
Wager-Smith, K;Kay, SA
通讯作者：
Kay, SA
影响因子：
9.8
作者：
Vatine G;Vallone D;Appelbaum L;Mracek P;Ben-Moshe Z;Lahiri K;Gothilf Y;Foulkes NS
通讯作者：
Foulkes NS