Polaprezinc (N-(3-aminopropionyl)-L-histidinato zinc) ameliorates dextran sulfate sodium-induced colitis in mice
Polaprezinc (N-(3-aminopropionyl)-L-histidinato zinc) ameliorates dextran sulfate sodium-induced colitis in mice
复制标题
Polaprezinc（N-（3-氨基丙酰基）-L-组氨酸锌）可改善葡聚糖硫酸钠诱导的小鼠结肠炎
DOI：
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发表时间：
2005
影响因子：
1.9
通讯作者：
M. Asaka
中科院分区：
文献类型：
--
作者：
T. Ohkawara;H. Takeda;Kanji Kato;K. Miyashita;Mototsugu Kato;T. Iwanaga;M. Asaka
客观的。 Polaprezinc（N-(3-氨基丙酰基)-L-组氨酸锌）是一种抗溃疡药物，据报道在多种炎症性疾病中具有抗炎作用。本研究的目的是研究聚普锌对硫酸葡聚糖 (DSS) 诱导的小鼠结肠炎的影响。材料和方法。 DSS诱导的结肠炎小鼠在给予DSS 7天后每天直肠内注射聚普锌（15 mg/kg）或硫酸锌（7.5 mg/kg）。评估疾病活动指数（DAI）和组织学组织损伤。测量结肠中髓过氧化物酶（MPO）活性、肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ的水平。通过蛋白质印迹分析分析结肠中热休克蛋白（HSP）25和HSP70的表达。结果。经聚普锌治疗的 DSS 诱导结肠炎小鼠的 DAI 和组织学评分显着降低。 Polaprezinc 可抑制 DSS 诱导的结肠炎小鼠结肠组织中 MPO 活性的增加以及 TNF-α 和 IFN-γ 的产生。在 DSS 治疗期间，polaprezinc 治疗的小鼠结肠组织中 HSP25 和 HSP70 的表达显着上调。结论。 Polaprezinc 可抑制 DSS 诱导的小鼠结肠炎，部分是通过抑制促炎细胞因子的产生、抑制中性粒细胞积累以及通过 HSP 过度表达来提供细胞保护。 Polaprezinc 可用于治疗炎症性肠病。
Objective. Polaprezinc (N-(3-Aminopropionyl)-L-histidinato zinc), an anti-ulcer drug, has been reported to have an anti-inflammatory action in several inflammatory diseases. The aim of this study was to investigate the effect of polaprezinc on dextran sulfate (DSS)-induced colitis in mice. Material and methods. Mice with colitis induced by DSS were intrarectally treated with polaprezinc (15 mg/kg) or zinc sulfate (7.5 mg/kg) every day after the administration of DSS for 7 days. Disease activity index (DAI) and histological tissue damage were assessed. Levels of myeloperoxidase (MPO) activity, tumor necrosis factor (TNF)-α and interferon (IFN)-γ in the colon were measured. Expression of heat shock protein (HSP) 25 and HSP70 in the colon was analyzed by Western blot analysis. Results. DAI and histological scores were remarkably reduced in polaprezinc-treated mice with DSS-induced colitis. Polaprezinc suppressed the increase of MPO activity and the production of TNF-α and IFN-γ in the colon tissues of mice with DSS-induced colitis. Expression of HSP25 and HSP70 was remarkably up-regulated in the colon tissues of polaprezinc-treated mice during DSS treatment. Conclusions. Polaprezinc suppresses DSS-induced colitis in mice, partly through inhibition of production of pro-inflammatory cytokine, suppression of neutrophils accumulation and cytoprotection by overexpression of HSPs. Polaprezinc could be useful in the treatment of inflammatory bowel diseases.
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影响因子：
29.4
作者：
Ropeleski, MJ;Tang, J;Chang, EB
通讯作者：
Chang, EB
影响因子：
29.4
作者：
Kojima, K;Musch, MW;Chang, EB
通讯作者：
Chang, EB
影响因子：
29.4
作者：
J. E. Krawisz;P. Sharon;W. Stenson
通讯作者：
J. E. Krawisz;P. Sharon;W. Stenson
影响因子：
15.9
作者：
MARBER, MS;MESTRIL, R;DILLMANN, WH
通讯作者：
DILLMANN, WH
影响因子：
29.4
作者：
H. Ren;M. Musch;K. Kojima;D. Boone;A. Ma;E. Chang
通讯作者：
H. Ren;M. Musch;K. Kojima;D. Boone;A. Ma;E. Chang