Nitric oxide-releasing S-nitrosothiol-modified xerogels.
Nitric oxide-releasing S-nitrosothiol-modified xerogels.
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DOI：
10.1016/j.biomaterials.2009.05.006
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Schoenfisch, Mark H.
中科院分区：
文献类型：
--
作者：
Riccio, Daniel A.;Dobmeier, Kevin P.;Hetrick, Evan M.;Privett, Benjamin J.;Paul, Heather S.;Schoenfisch, Mark H.
描述了 S-亚硝基硫醇 (RSNO) 修饰干凝胶的合成、材料表征和体外生物相容性。硫醇官能化干凝胶薄膜是通过不同浓度的 3-巯基丙基三甲氧基硅烷 (MPTMS) 和甲基三甲氧基硅烷 (MTMOS) 溶胶-凝胶前体的水解和共缩合形成的。随后通过酸化亚硝酸盐进行硫醇亚硝化，产生 RSNO 修饰的干凝胶，能够在生理条件下产生一氧化氮 (NO) 长达 2 周。干凝胶在广谱光照射或接触铜时也表现出 NO 生成，NO 通量分别与瓦数和浓度成正比。干凝胶能够储存高达~1.31 µmol NO mg−1，并且在两周内显示出可忽略不计的碎片。与非亚硝化对照相比，亚硝化膜上的血小板和细菌粘附减少，证实了释放 NO 的材料的抗血栓和抗菌特性。干凝胶表面上的成纤维细胞活力得以保持，这说明 RSNO 修饰的干凝胶作为生物医学设备涂层的前景。
The synthesis, material characterization, and in vitro biocompatibility of S-nitrosothiol (RSNO)-modified xerogels is described. Thiol-functionalized xerogel films were formed by hydrolysis and co-condensation of 3-mercaptopropyltrimethoxysilane (MPTMS) and methyltrimethoxysilane (MTMOS) sol-gel precursors at varying concentrations. Subsequent thiol nitrosation via acidified nitrite produced RSNO-modified xerogels capable of generating nitric oxide (NO) for up to 2 weeks under physiological conditions. Xerogels also exhibited NO generation upon irradiation with broad-spectrum light or exposure to copper, with NO fluxes proportional to wattage and concentration, respectively. Xerogels were capable of storing up to ∼1.31 µmol NO mg−1, and displayed negligible fragmentation over a 2 week period. Platelet and bacterial adhesion to nitrosated films was reduced compared to non-nitrosated controls, confirming the antithrombotic and antibacterial properties of the NO-releasing materials. Fibroblast cell viability was maintained on the xerogel surfaces illustrating the promise of RSNO-modified xerogels as biomedical device coatings.
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DOI：
10.1039/p29960000481
发表时间：
1996-04-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
影响因子：
--
作者：
Dicks, AP;Swift, HR;Cox, BG
通讯作者：
Cox, BG
影响因子：
14
作者：
Hwang, Sangyeul;Meyerhoff, Mark E.
通讯作者：
Meyerhoff, Mark E.
影响因子：
168.9
作者：
LANGFORD, EJ;BROWN, AS;MONCADA, S
通讯作者：
MONCADA, S
影响因子：
56.9
作者：
GRISTINA, AG
通讯作者：
GRISTINA, AG
影响因子：
14
作者：
Hetrick, Evan M.;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.