Comparison of three gamma oscillations in the mouse entorhinal-hippocampal system.
Comparison of three gamma oscillations in the mouse entorhinal-hippocampal system.
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DOI：
10.1111/ejn.13831
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Paulsen O
中科院分区：
文献类型：
--
作者：
Butler JL;Hay YA;Paulsen O
内嗅-海马体系统是大脑中一个重要的回路，对记忆和导航等某些认知任务至关重要。不同的伽马振荡发生在这个回路中，内侧内嗅皮层（mEC）、CA3和CA1都产生不同性质的伽马振荡。这三种伽马振荡在CA1中收敛，在那里已经做了很多工作来试图将它们彼此隔离开来。在这里，我们比较了mEC， CA3和CA1中的伽马发生器，使用光遗传诱导的theta-gamma振荡。在这三个区域的主要神经元中表达通道视紫红质- 2可以通过正弦蓝光刺激在θ频率下诱导伽马振荡。记录体内CA1的振荡，我们发现CA3刺激诱导的伽马振荡比CA1刺激诱导的慢，这与体内自发CA3和CA1伽马振荡的报道相匹配。在离体脑切片中，CA3的光遗传刺激诱导的伽马振荡比mEC或CA1的刺激慢，两者的伽马振荡频率相似。所有三种伽马振荡在同一区域的树突层和树突层之间都有一个电流汇源对。利用该模型在切片中分析伽马频率机制，我们利用药理学证明了所有三种伽马振荡都依赖于相同类型的突触受体，通过阻断A型γ -氨基丁酸受体或α -氨基- 3 -羟基- 5 -甲基- 4 -异唑油酸/海因酸受体而被消除，并且对N -甲基- d -天冬氨酸受体的阻断不敏感。这些结果表明，一个快速的兴奋-抑制反馈回路是所有三个区域产生伽马振荡的基础。
The entorhinal–hippocampal system is an important circuit in the brain, essential for certain cognitive tasks such as memory and navigation. Different gamma oscillations occur in this circuit, with the medial entorhinal cortex (mEC), CA3 and CA1 all generating gamma oscillations with different properties. These three gamma oscillations converge within CA1, where much work has gone into trying to isolate them from each other. Here, we compared the gamma generators in the mEC, CA3 and CA1 using optogenetically induced theta–gamma oscillations. Expressing channelrhodopsin‐2 in principal neurons in each of the three regions allowed for the induction of gamma oscillations via sinusoidal blue light stimulation at theta frequency. Recording the oscillations in CA1 in vivo, we found that CA3 stimulation induced slower gamma oscillations than CA1 stimulation, matching in vivo reports of spontaneous CA3 and CA1 gamma oscillations. In brain slices ex vivo, optogenetic stimulation of CA3 induced slower gamma oscillations than stimulation of either mEC or CA1, whose gamma oscillations were of similar frequency. All three gamma oscillations had a current sink–source pair between the perisomatic and dendritic layers of the same region. Taking advantage of this model to analyse gamma frequency mechanisms in slice, we showed using pharmacology that all three gamma oscillations were dependent on the same types of synaptic receptor, being abolished by blockade of either type A γ‐aminobutyric acid receptors or α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid/kainate receptors, and insensitive to blockade of N‐methyl‐d‐aspartate receptors. These results indicate that a fast excitatory–inhibitory feedback loop underlies the generation of gamma oscillations in all three regions.
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影响因子：
16.2
作者：
Fries P
通讯作者：
Fries P
影响因子：
3.5
作者：
Mably, Alexandra J.;Gereke, Brian J.;Colgin, Laura Lee
通讯作者：
Colgin, Laura Lee
影响因子：
5.3
作者：
Morita, Kenji;Kalra, Rita;Robinson, Hugh P. C.
通讯作者：
Robinson, Hugh P. C.
DOI：
10.1523/jneurosci.4122-11.2012
发表时间：
2012-01-11
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Belluscio MA;Mizuseki K;Schmidt R;Kempter R;Buzsáki G
通讯作者：
Buzsáki G
DOI：
10.1111/ejn.13582
发表时间：
2017-06
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Betterton RT;Broad LM;Tsaneva-Atanasova K;Mellor JR
通讯作者：
Mellor JR