An antisense RNA that governs the expression kinetics of a multifunctional virulence gene.
An antisense RNA that governs the expression kinetics of a multifunctional virulence gene.
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DOI：
10.1111/j.1365-2958.2010.07161.x
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发表时间：
2010-05
影响因子：
3.6
通讯作者：
Groisman EA
中科院分区：
文献类型：
--
作者：
Lee EJ;Groisman EA
最近，对几种细菌的全基因组转录组分析发现了迄今为止未被重视的反义转录量。然而，这种反义转录物的生理作用、调节和意义目前尚不清楚。我们现在报告鉴定的顺式编码的1.2 kb长的反义RNA -称为AmgR -这是互补的mgtC部分的mgtCBR多顺反子消息从沙门氏菌肠道。mgtCBR mRNA指定MgtC蛋白，其对于巨噬细胞内的存活、小鼠中的毒力和在低Mg 2+中的生长是必需的; Mg 2+转运蛋白MgtB，其在毒力中没有明显作用;以及膜肽MgtR介导MgtC降解。AmgR的表达在需要RNase E但独立于RNase III、RNA伴侣Hfq和调节肽MgtR的过程中降低了MgtC和MgtB蛋白水平。染色体amgR启动子的失活增加了MgtC和MgtB蛋白水平，并增强了沙门氏菌的毒力。令人惊讶的是，AmgR转录受调控蛋白PhoP控制，PhoP也指导正义mgtCBR mRNA的转录。AmgR可能作为一个定时装置，改变MgtC和MgtB水平后，PhoP诱导条件的发作。
Genome-wide transcriptome analyses of several bacterial species have recently uncovered a hitherto unappreciated amount of antisense transcription. However, the physiological role, regulation and significance of such antisense transcripts are presently unclear. We now report the identification of a cis-encoded 1.2 kb long antisense RNA – termed AmgR – that is complementary to the mgtC portion of the mgtCBR polycistronic message from Salmonella enterica. The mgtCBR mRNA specifies the MgtC protein, which is necessary for survival within macrophages, virulence in mice and growth in low Mg2+; the Mg2+ transporter MgtB with no apparent role in virulence; and the membrane peptide MgtR mediating MgtC degradation. Expression of AmgR dimished both MgtC and MgtB protein levels in a process requiring RNase E but independent of RNase III, the RNA chaperone Hfq, and the regulatory peptide MgtR. Inactivation of the chromosomal amgR promoter increased MgtC and MgtB protein levels and enhanced Salmonella virulence. Surprisingly, AmgR transcription is governed by the regulatory protein PhoP, which also directs transcription of the sense mgtCBR mRNA. AmgR may function as a timing device that alters MgtC and MgtB levels after the onset of PhoP-inducing conditions.
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影响因子：
11.4
作者：
Alix, Eric;Blanc-Potard, Anne-Beatrice
通讯作者：
Blanc-Potard, Anne-Beatrice
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
Eiamphungporn, Warawan;Helmann, John D.
通讯作者：
Helmann, John D.
影响因子：
3.6
作者：
Bougdour, Alexandre;Cunning, Christofer;Gottesman, Susan
通讯作者：
Gottesman, Susan
影响因子：
3.6
作者：
Buchmeier, N;Blanc-Potard, A;Groisman, EA
通讯作者：
Groisman, EA