Regulatory B cells in allergic airways disease and asthma.

Regulatory B cells in allergic airways disease and asthma.
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过敏性气道疾病和哮喘中的调节性 B 细胞。

DOI:
10.1007/978-1-4939-1161-5_15
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发表时间:
2014
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Schramm,CraigM
Schramm,CraigM
中科院分区:
--
文献类型:
--
作者:
Natarajan,Prabitha;Guernsey,LindaA;Schramm,CraigM

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B细胞通过促进抗原呈递和通过产生抗原特异性抗体而参与先天性和适应性免疫应答。最近的证据表明,某些B细胞亚群也可以抑制T细胞免疫反应。与调节性T细胞(Treg)一样,这些调节性B细胞(布雷格)似乎包含几个亚群。布雷格细胞是如何产生的,以及它们如何控制体内的免疫反应,才刚刚开始被阐明。在这里,我们提供了详细的说明,识别,分离和功能特性的布雷格细胞在小鼠模型的过敏性气道疾病。
B cells have long been known to participate in both innate and adaptive immune responses by contributing to antigen presentation and by producing antigen-specific antibodies. Recent evidence shows that certain B-cell subsets can also inhibit T-cell immune responses. Like regulatory T cells (Treg), these regulatory B cells (Breg) appear to comprise several subpopulations. How Breg cells are generated and how they control immune responses in vivo are just beginning to be elucidated. Here, we provide detailed instructions for the identification, isolation, and functional characterization of Breg cells in a murine model of allergic airway disease.
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