Durable and stratum-specific gene expression in epidermis.
Durable and stratum-specific gene expression in epidermis.
复制标题
表皮中持久且特定层的基因表达。
DOI：
10.1038/sj.gt.3301800
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发表时间：
2002
期刊：
影响因子：
5.1
通讯作者：
Taichman,LB
中科院分区：
文献类型：
--
作者：
Ghazizadeh,S;Doumeng,C;Taichman,LB
A number of genetic disorders are manifested in cutaneous epithelium and gene therapy approaches for treatment of such diseases are being considered. A successful gene therapy protocol requires durable and correctly targeted gene expression within the tissue. The continuous renewal and high levels of compartmentalization in epidermis are two challenges for a successful gene therapy of skin disorders. For those disorders which affect the upper layers of epidermis, vectors must be available that target stem cells, but remain silent until the progeny of these cells undergo differentiation. To explore the potential of long-term and targeted vector expression in epidermis, a hybrid retroviral vector encoding the reporter enhanced green fluorescent protein (EGFP) was constructed. The viral enhancer in the long terminal repeat of the vector was replaced with a 510-bp enhancer element of the human involucrin promoter. Keratinocyte-specific expression directed by the hybrid vector was demonstrated in culture and suprabasal-specific expression was observed in organotypic human epidermal cultures. In vivo transduction of mouse skin with this hybrid vector indicated long-term and stratum-specific expression of the transgene in mouse epidermis. The design of similar vectors for various gene therapy applications constitutes an important step toward clinically effective gene therapy.
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通过施用左旋多巴逆转多巴胺受体超敏性的两种表现。
DOI：
--
发表时间：
1977
期刊：
Research communications in chemical pathology and pharmacology
影响因子：
--
作者：
A. Friedhoff;K. Bonnet;H. Tosengarten
通讯作者：
H. Tosengarten
影响因子：
--
作者：
D. Jeste;R. Wyatt
通讯作者：
R. Wyatt
溴隐亭和麦角曲治疗帕金森病期间的精神障碍。
影响因子：
17.7
作者：
M. Serby;B. Angrist;A. Lieberman
通讯作者：
A. Lieberman
大剂量溴隐亭治疗迟发性肌张力障碍一例
影响因子：
10.6
作者：
D. Luchins;M. Goldman
通讯作者：
M. Goldman
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影响因子：
--
作者：
C. Tamminga;T. Chase
通讯作者：
T. Chase