Segmental Additive Tissue Engineering.
Segmental Additive Tissue Engineering.
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DOI：
10.1038/s41598-018-29270-4
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发表时间：
2018-07-18
影响因子：
4.6
通讯作者：
de Peppo GM
中科院分区：
文献类型：
--
作者：
Sladkova M;Alawadhi R;Jaragh Alhaddad R;Esmael A;Alansari S;Saad M;Mulla Yousef J;Alqaoud L;de Peppo GM
由创伤和疾病引起的节段性骨缺损是世界范围内的一个主要临床问题。目前的治疗选择有限，并且常常导致不良结果和严重并发症。骨工程是一种有前途的替代解决方案，但必须解决许多技术挑战，才能有效且可重复地构建分段移植物，满足个体患者和常规临床应用所需的尺寸和几何要求。设计工程策略和标准操作程序非常重要，从而可以扩大骨工程移植物的尺寸，最大限度地减少工艺和产品的可变性，并促进技术转让和实施。为了解决这些问题，我们将传统和模块化组织工程方法结合在称为分段增材组织工程（SATE）的策略中。为了证明这种方法，兔股骨缺损的数字重建被横向于纵轴分割成具有盘状几何形状和定义厚度的片段（模块），以实现协议标准化和体外有效组织形成。然后使用接种有人类诱导多能干细胞衍生的中胚层祖细胞（iPSC-MP）的仿生支架和具有通用设计的新型灌注生物反应器来设计与每个节段相对应的骨移植物。 SATE战略能够实现有效且可重复的节段骨移植工程，以实现个性化骨骼重建，并将促进节段骨缺损治疗的组织工程方法的技术转让和实施。
Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenges must be addressed to allow for effective and reproducible construction of segmental grafts that meet the size and geometrical requirements needed for individual patients and routine clinical applications. It is important to devise engineering strategies and standard operating procedures that make it possible to scale up the size of bone-engineered grafts, minimize process and product variability, and facilitate technology transfer and implementation. To address these issues, we have combined traditional and modular tissue engineering approaches in a strategy referred to as Segmental Additive Tissue Engineering (SATE). To demonstrate this approach, a digital reconstruction of a rabbit femoral defect was partitioned transversally to the longitudinal axis into segments (modules) with discoidal geometry and defined thickness to enable protocol standardization and effective tissue formation in vitro. Bone grafts corresponding to each segment were then engineered using biomimetic scaffolds seeded with human induced pluripotent stem cell-derived mesodermal progenitors (iPSC-MPs) and a novel perfusion bioreactor with universal design. The SATE strategy enables the effective and reproducible engineering of segmental bone grafts for personalized skeletal reconstruction, and will facilitate technology transfer and implementation of a tissue engineering approach to segmental bone defect therapy.
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影响因子：
10
作者：
Hinderliter PM;Minard KR;Orr G;Chrisler WB;Thrall BD;Pounds JG;Teeguarden JG
通讯作者：
Teeguarden JG
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7.8
作者：
Zhou, Shuanhu;Greenberger, Joel S.;Glowacki, Julie
通讯作者：
Glowacki, Julie
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Tanabe, Koji;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
影响因子：
46.9
作者：
Petite, H;Viateau, V;Guillemin, G
通讯作者：
Guillemin, G