Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation.
Deciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation.
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解剖梭状芽胞杆菌代谢及其对合成剂发酵过程中对生物反应器传质的反应。
DOI：
10.1038/s41598-017-10312-2
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发表时间：
2017-08-30
影响因子：
4.6
通讯作者：
Wen Z
中科院分区：
文献类型：
--
作者：
Wan N;Sathish A;You L;Tang YJ;Wen Z
This study used 13C tracers and dynamic labeling to reveal metabolic features (nutrients requirements, pathway delineation and metabolite turnover rates) of Clostridium carboxidivorans P7, a model strain for industrial syngas fermentation, and its implication with bioreactor mass transfer. P7 shows poor activity for synthesizing amino acids (e.g., phenylalanine) and thus, needs rich medium for cell growth. The strain has multiple carbon fixation routes (Wood-Ljungdahl pathway, pyruvate:ferredoxin oxidoreductase reaction and anaplerotic pathways) and Re-citrate synthase (Ccar_06155) was a key enzyme in its tricarboxylic acid cycle (TCA) pathway. High fluxes were observed in P7’s Wood-Ljungdahl pathway, right branch of TCA cycle, pyruvate synthesis, and sugar phosphate pathways, but the cells anabolic pathways were strikingly slow. In bioreactor culture, when syngas flowrate increased from 1 to 10 mL/min, P7 strain produced same amount of total extracellular products (acids and alcohols) but high flowrate favored alcohol accumulation. This observation was due to the mass transfer limitation influencing energy metabolism (CO/H2 oxidation for cofactor generations) more prominently than carbon fixation. When syngas flowrate increased from 10 of 20 mL/min, the alcohol productivity was not improved and the labeling rate (~0.03 h−1) of key metabolite acetyl-CoA reached to P7 strain’s metabolism limitation regime.
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影响因子：
4.4
作者：
Feng, Xueyang;Mouttaki, Housna;Tang, Yinjie J.
通讯作者：
Tang, Yinjie J.
影响因子：
3.7
作者：
Bruant G;Lévesque MJ;Peter C;Guiot SR;Masson L
通讯作者：
Masson L
影响因子：
11.4
作者：
Bosma, TNP;Middeldorp, PJM;Zehnder, AJB
通讯作者：
Zehnder, AJB
影响因子：
5
作者：
Fernandez-Naveira, Anxela;Nalakath Abubackar, Haris;Kennes, Christian
通讯作者：
Kennes, Christian
影响因子：
7.3
作者：
HAPPEL, J;WALTER, E;LECOURTIER, Y
通讯作者：
LECOURTIER, Y