Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases.
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases.
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DOI：
10.1038/nbt1409
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发表时间：
2008-06
影响因子：
46.9
通讯作者：
Amacher, Sharon L.
中科院分区：
文献类型：
--
作者：
Doyon, Yannick;McCammon, Jasmine M.;Miller, Jeffrey C.;Faraji, Farhoud;Ngo, Catherine;Katibah, George E.;Amora, Rainier;Hocking, Toby D.;Zhang, Lei;Rebar, Edward J.;Gregory, Philip D.;Urnov, Fyodor D.;Amacher, Sharon L.
在这里，我们描述了使用锌指核酸酶（ZFN）的体细胞和生殖细胞的基因在斑马鱼（Danio rerio），其中有针对性的诱变是以前棘手的中断。ZFN诱导基因组中的靶向双链断裂，其被修复以产生小的插入和缺失。我们设计了靶向斑马鱼golden和无尾/Brachyury基因的ZFN。在这两种情况下，将ZFN编码mRNA注射到1-细胞胚胎中产生了高百分比的动物，这些动物在ZFN指定的位置携带不同的突变，并表现出预期的功能丧失表型。破坏的ntl等位基因从注射ZFN mRNA的建立者动物以平均20%的频率在超过一半的测试成年动物中传播。观察到的基因破坏事件的频率和精确度，结合针对任何位点设计ZFN的能力，从根本上开辟了新的实验途径，并表明ZFN技术可以广泛应用于许多允许mRNA递送到受精卵中的生物体。
We describe here the use of zinc finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), where targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury genes. In both cases, injection of ZFN-encoding mRNA into 1-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Disrupted ntl alleles were transmitted from ZFN mRNA-injected founder animals in over half the adults tested at frequencies averaging 20%. The frequency and precision of gene disruption events observed, in combination with the ability to design ZFNs against any locus, open fundamentally novel avenues of experimentation, and suggest that ZFN technology may be widely applied to many organisms that allow mRNA delivery into the fertilized egg.
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DOI：
10.1073/pnas.0800940105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Santiago, Yolanda;Chan, Edmond;Collingwood, Trevor N.
通讯作者：
Collingwood, Trevor N.
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
2.7
作者：
STREISINGER, G;COALE, F;GRUNWALD, DJ
通讯作者：
GRUNWALD, DJ
影响因子：
64.5
作者：
THOMAS, KR;FOLGER, KR;CAPECCHI, MR
通讯作者：
CAPECCHI, MR
影响因子：
64.8
作者：
Urnov, FD;Miller, JC;Holmes, MC
通讯作者：
Holmes, MC