Genomic analysis of a Mycobacterium bovis bacillus [corrected] Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis.
Genomic analysis of a Mycobacterium bovis bacillus [corrected] Calmette-Guérin strain isolated from an adult patient with pulmonary tuberculosis.
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从成年患有肺结核的成年患者分离的牛肉杆菌[校正]的牛肉杆菌的基因组分析。
DOI：
10.1371/journal.pone.0122403
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Li X;Chen L;Zhu Y;Yu X;Cao J;Wang R;Lv X;He J;Guo A;Huang H;Zheng H;Liu S
多年来，卡介苗（BCG）一直是唯一的结核病疫苗，通常被认为是安全的。然而，从结核病患者分离的标记为3281的临床菌株被鉴定为BCG。通过下一代测序（NGS）和比较基因组分析的结合，揭示了3281个独特的遗传特征。发现与初始染色体复制密切相关的包含dnaA和dnaN基因的区域在BCG巴斯德特异性串联重复区域DU1上重复三次。由于BCG菌株中的表位数量最少，因此推断3281具有较高的免疫逃避可能性。此外，分析了毒力基因的变异和潜在毒力因子的预测。总的来说，我们报告了一种以前从未被认为是致病性的病原体，并初步了解了毒性BCG的遗传特征。
For years, bacillus Calmette-Guérin (BCG) has served as the unique vaccine against tuberculosis and has generally been regarded as safe. However, a clinical strain labeled 3281 that was isolated from a TB patient was identified to be BCG. Via the combination of next-generation sequencing (NGS) and comparative genomic analysis, unique 3281 genetic characteristics were revealed. A region containing the dnaA and dnaN genes that is closely related to the initial chromosome replication was found to repeat three times on the BCG Pasteur-specific tandem duplication region DU1. Due to the minimum number of epitopes in BCG strains, 3281 was inferred to have a high possibility for immune evasion. Additionally, variations in the virulence genes and predictions for potential virulence factors were analyzed. Overall, we report a pathogen that has never previously been thought to be pathogenic and initial insights that are focused on the genetic characteristics of virulent BCG.
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