Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin.
Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin.
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DOI：
10.1155/2011/981793
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发表时间：
2011
影响因子：
--
通讯作者：
Turkeli M
中科院分区：
文献类型：
--
作者：
Karadeniz A;Simsek N;Karakus E;Yildirim S;Kara A;Can I;Kisa F;Emre H;Turkeli M
顺铂（CDDP）是肿瘤治疗中最活跃的细胞毒性药物之一，具有肾毒性和肝毒性等不良副作用。本研究旨在通过测定组织生化指标、抗氧化指标和细胞凋亡免疫组织化学方法，探讨蜂王浆（RJ）对CDDP损伤所致肾、肝氧化应激的影响。24只Sprague Dawley大鼠分为4组，C组：对照组给予0.9%生理盐水；CDDP组：腹腔注射顺铂（CDDP， 7 mg kg - 1体重腹腔注射，单次给药）；RJ组：连续15 d灌胃RJ (300 mg/kg/d)；RJ + CDDP组：单次注射CDDP后15 d灌胃RJ。测定肝脏和肾脏匀浆中丙二醛（MDA）和谷胱甘肽（GSH）水平、谷胱甘肽s转移酶（GST）、谷胱甘肽过氧化物酶（GSH- px）和超氧化物歧化酶（SOD）活性，并对肝脏和肾脏进行组织学检查。RJ通过降低脂质过氧化（MDA）水平，提高GSH水平，提高GST、GSH- px和SOD活性，对肝、肾具有显著的保护作用。免疫组化检查显示，顺铂组小鼠的凋亡细胞数量和退行性改变明显增加，而RJ + CDDP组小鼠的肝、肾组织的这些组织学改变较顺铂组明显减少。此外，RJ治疗导致肝细胞和小管上皮抗凋亡活性增加。综上所述，RJ联合顺铂可改善顺铂诱导的氧化应激参数和细胞凋亡活性。
Cisplatin (CDDP) is one of the most active cytotoxic agents in the treatment of cancer and has adverse side effects such as nephrotoxicity and hepatotoxicity. The present study was designed to determine the effects of royal jelly (RJ) against oxidative stress caused by CDDP injury of the kidneys and liver, by measuring tissue biochemical and antioxidant parameters and investigating apoptosis immunohistochemically. Twenty-four Sprague Dawley rats were divided into four groups, group C: control group received 0.9% saline; group CDDP: injected i.p. with cisplatin (CDDP, 7 mg kg−1 body weight i.p., single dose); group RJ: treated for 15 consecutive days by gavage with RJ (300 mg/kg/day); group RJ + CDDP: treated by gavage with RJ 15 days following a single injection of CDDP. Malondialdehyde (MDA) and glutathione (GSH) levels, glutathione S-transferase (GST), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activities were determined in liver and kidney homogenates, and the liver and kidney were also histologically examined. RJ elicited a significant protective effect towards liver and kidney by decreasing the level of lipid peroxidation (MDA), elevating the level of GSH, and increasing the activities of GST, GSH-Px, and SOD. In the immunohistochemical examinations were observed significantly enhanced apoptotic cell numbers and degenerative changes by cisplatin, but these histological changes were lower in the liver and kidney tissues of RJ + CDDP group. Besides, treatment with RJ lead to an increase in antiapoptotic activity hepatocytes and tubular epithelium. In conclusion, RJ may be used in combination with cisplatin in chemotherapy to improve cisplatin-induced oxidative stress parameters and apoptotic activity.
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影响因子：
19.6
作者：
Baliga, R;Zhang, ZW;Shah, SV
通讯作者：
Shah, SV
影响因子：
19.6
作者：
Lau, AH
通讯作者：
Lau, AH
影响因子：
2.2
作者：
Khan E;Batuman V;Lertora JJ
通讯作者：
Lertora JJ
影响因子：
4.3
作者：
Cemek, Mustafa;Aymelek, Fatih;Yilmaz, Fatma
通讯作者：
Yilmaz, Fatma
影响因子：
--
作者：
Cayir, Kerim;Karadeniz, Ali;Tekin, Salim Basol
通讯作者：
Tekin, Salim Basol