Evaluation of spectral photon counting computed tomography K-edge imaging for determination of gold nanoparticle biodistribution in vivo.
Evaluation of spectral photon counting computed tomography K-edge imaging for determination of gold nanoparticle biodistribution in vivo.
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DOI：
10.1039/c7nr01153a
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发表时间：
2017-11-30
期刊：
影响因子：
6.7
通讯作者：
Douek P
中科院分区：
文献类型：
--
作者：
Si-Mohamed S;Cormode DP;Bar-Ness D;Sigovan M;Naha PC;Langlois JB;Chalabreysse L;Coulon P;Blevis I;Roessl E;Erhard K;Boussel L;Douek P
光谱光子计数计算机断层成像（SPCCT）是一种新兴的医学成像技术。SPCCT扫描仪记录入射光子的能量，这允许由于测量其特征X射线衰减曲线而对造影剂进行特定检测。这种方法被称为K边缘成像。由元素如金、铋或镱形成的纳米颗粒已被报道为用于SPCCT成像的潜在造影剂。此外，金纳米粒子在医学上有许多应用，例如用于放射治疗和光热消融的佐剂。纳米颗粒的生物分布的特异性纵向成像对于其临床转化具有高度吸引力。因此，我们研究了一种新的SPCCT扫描仪的能力，以量化体内的金纳米粒子的生物分布。使用PEG化的金纳米颗粒。体模成像显示，在金图像上测量的浓度与已知浓度相关性良好（斜率= 0.94，截距= 0.18，RMSE = 0.18，R2 = 0.99）。SPCCT系统允许在体内重复和快速采集，并随时间跟踪AuNP生物分布的变化。在金图像上进行的测量与感兴趣器官中的电感耦合等离子体发射光谱法（ICP-OES）测量相关（斜率= 0. 77，截距= 0. 47，RMSE = 0. 72，R2 = 0. 93）。TEM与成像和ICP-OES一致，因为在肝、脾、骨髓和淋巴结（主要在巨噬细胞中）中观察到高得多的AuNP浓度。总之，我们发现SPCCT能够重复和非侵入性地测定金纳米颗粒在体内的生物分布。一种新的光谱光子计数CT原型具有随时间推移无创定量测定金纳米颗粒在体内生物分布的潜力。
Spectral photon counting computed tomography (SPCCT) is an emerging medical imaging technology. SPCCT scanners record the energy of incident photons, which allows specific detection of contrast agents due to measurement of their characteristic x-ray attenuation profiles. This approach is known as K-edge imaging. Nanoparticles formed from elements such as gold, bismuth or ytterbium have been reported as potential contrast agents for SPCCT imaging. Furthermore, gold nanoparticles have many applications in medicine, such as adjuvants for radiotherapy and photothermal ablation. Specific, longitudinal imaging of the biodistribution of nanoparticles would be highly attractive for their clinical translation. We therefore studied the capabilities of a novel SPCCT scanner to quantify the biodistribution of gold nanoparticles in vivo. PEGylated gold nanoparticles were used. Phantom imaging showed that concentrations measured on gold images correlated well with known concentrations (slope = 0.94, intercept = 0.18, RMSE = 0.18, R2 = 0.99). The SPCCT system allowed repetitive and quick acquisitions in vivo, and follow-up of changes in the AuNP biodistribution over time. Measurements performed on gold images correlated with the Inductively coupled plasma-optical emission spectrometry (ICP-OES) measurements in the organs of interest (slope = 0.77, intercept = 0.47, RMSE = 0.72, R2 = 0.93). TEM agreed with the imaging and ICP-OES in that much higher concentrations of AuNP were observed in the liver, spleen, bone marrow and lymph nodes (mainly in macrophages). In conclusion, we found that SPCCT is capable of repetitive and non invasive determination of the biodistribution of gold nanoparticles in vivo. A new spectral photon-counting CT prototype has the potentiel for non-invasive quantitative determination of gold nanoparticle biodistribution in vivo over time.
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影响因子：
5
作者：
Grosjean, Romain;Sauer, Benoit;Hubert, Jacques
通讯作者：
Hubert, Jacques
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FitzGerald, Paul F.;Colborn, Robert E.;Yeh, Benjamin M.
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