Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice.
Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice.
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DOI：
10.1093/pcp/pcn123
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发表时间：
2008-10
影响因子：
4.9
通讯作者：
Matsuoka M
中科院分区：
文献类型：
--
作者：
Hirano K;Aya K;Hobo T;Sakakibara H;Kojima M;Shim RA;Hasegawa Y;Ueguchi-Tanaka M;Matsuoka M
为探讨植物激素在水稻小孢子/花粉（MS/POL）发育过程中的作用，测定了成熟花药中内源激素IAA、赤霉素（GAs）、细胞分裂素（CKs）和脱落酸（阿坝）的含量。我们还分析了与7种植物激素，即生长素，GAs，CKs，油菜素类固醇，乙烯，阿坝和茉莉酸，在MS/POL和绒毡层（TAP）的基因的全球表达谱使用44 K微阵列结合激光显微切割技术（LM阵列分析）。IAA和GA_4在成熟花药中的积累量明显高于其它组织，而CK_s和阿坝在成熟花药中的积累量不明显。LM-阵列分析表明，在MS/POL发育的后期，IAA和GA合成所需的基因组协调表达，表明这些基因负责成熟花药中IAA和GA 4的大量积累。与此相反，GA信号的基因优先在MS/POL和整个TAP发展的早期发展阶段的表达，而他们的表达在MS/POL发展的后期阶段下调。在生长素信号基因的情况下，在GA合成和信号基因中观察到的这种镜像表达没有观察到。IAA受体基因主要在MS/POL发育的后期表达，AUX/IAA和ARF基因在MS/POL和TAP发育的不同时期表达。这种植物激素生物合成和信号传导基因的细胞类型特异性表达谱证明了独立于其他细胞/组织分析单个细胞类型中植物激素相关基因表达的有效性和重要性。
To investigate the involvement of phytohormones during rice microspore/pollen (MS/POL) development, endogenous levels of IAA, gibberellins (GAs), cytokinins (CKs) and abscisic acid (ABA) in the mature anther were analyzed. We also analyzed the global expression profiles of genes related to seven phytohormones, namely auxin, GAs, CKs, brassinosteroids, ethylene, ABA and jasmonic acids, in MS/POL and tapetum (TAP) using a 44K microarray combined with a laser microdissection technique (LM-array analysis). IAA and GA4 accumulated in a much higher amount in the mature anther compared with the other tissues, while CKs and ABA did not. LM-array analysis revealed that sets of genes required for IAA and GA synthesis were coordinately expressed during the later stages of MS/POL development, suggesting that these genes are responsible for the massive accumulation of IAA and GA4 in the mature anther. In contrast, genes for GA signaling were preferentially expressed during the early developmental stages of MS/POL and throughout TAP development, while their expression was down-regulated at the later stages of MS/POL development. In the case of auxin signaling genes, such mirror-imaged expression observed in GA synthesis and signaling genes was not observed. IAA receptor genes were mostly expressed during the late stages of MS/POL development, and various sets of AUX/IAA and ARF genes were expressed during the different stages of MS/POL or TAP development. Such cell type-specific expression profiles of phytohormone biosynthesis and signaling genes demonstrate the validity and importance of analyzing the expression of phytohormone-related genes in individual cell types independently of other cells/tissues.
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影响因子：
7.4
作者：
Agrawal, GK;Yamazaki, M;Hirochika, H
通讯作者：
Hirochika, H
影响因子：
11.6
作者：
Dombrecht, Bruno;Xue, Gang Ping;Kazan, Kemal
通讯作者：
Kazan, Kemal
影响因子：
4.9
作者：
Ariizumi, Tohru;Kawanabe, Takahiro;Toriyama, Kinya
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Toriyama, Kinya
影响因子：
11.6
作者：
Gosti, F;Beaudoin, N;Giraudat, J
通讯作者：
Giraudat, J
影响因子：
11.6
作者：
Cecchetti, Valentina;Altamura, Maria Maddalena;Cardarelli, Maura
通讯作者：
Cardarelli, Maura