Tandem mass tag-based quantitative proteomics reveals the regulators in biofilm formation and biofilm control of Bacillus licheniformis

Tandem mass tag-based quantitative proteomics reveals the regulators in biofilm formation and biofilm control of Bacillus licheniformis
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基于串联质量标签的定量蛋白质组学揭示了地衣芽孢杆菌生物膜形成和生物膜控制的调节因子

DOI:
10.1016/j.foodcont.2019.107029
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发表时间:
2020-04
期刊:
影响因子:
6
通讯作者:
Yuan Lei
Yuan Lei
中科院分区:
农林科学1区
文献类型:
--
作者:
Wang Ni;Sadiq Faizan Ahmed;Li Shanshan;He Guoqing;Yuan Lei

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地衣芽孢杆菌是乳品工业中一种具有很强腐败能力的主要污染物,其生物膜形成和生物膜控制的调控机制有待研究。本研究采用串联质谱标记的定量蛋白质组学方法,比较了生物膜细胞和大肠杆菌增殖细胞的表达谱。地衣状。基质的产生和芽孢的形成、细菌的趋化性、鞭毛的组装和双组分系统对B.地衣状。显著上调的Spo0F和KapB蛋白以及下调的MotB、FliG和FliK蛋白是植物乳杆菌生物膜预防的关键调节因子。蛋白质组学图谱为研究生物膜的形成和控制提供了新的视角。地衣状。
Regulatory mechanisms ofBacillus licheniformisbiofilm formation and biofilm control are desired to be explored as it is a major contaminant with strong spoilage capacity in the dairy industry. In this study, tandem mass tag-based quantitative proteomics was employed to compare the expression profiles between biofilm cells and planktonic cells ofB. licheniformis. Matrix production and sporulation, bacterial chemotaxis, flagellar assembly and two-component system played important roles on biofilm formation byB. licheniformis. Significant up-regulation proteins Spo0F and KapB, as well as down-regulation proteins MotB, FliG and FliK are the key regulators for biofilm prevention byLactobacillus plantarum. The proteomics profiles provide novel insights into the biofilm formation and control ofB. licheniformis.
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