Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.
Glycosaminoglycans compositional analysis of Urodele axolotl (Ambystoma mexicanum) and Porcine Retina.
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DOI：
10.1007/s10719-019-09863-5
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发表时间：
2019-04
影响因子：
3
通讯作者：
Linhardt RJ
中科院分区：
文献类型：
--
作者：
Kim SY;Kundu J;Williams A;Yandulskaya AS;Monaghan JR;Carrier RL;Linhardt RJ
视网膜退行性疾病，如年龄相关性黄斑变性（AMD）和视网膜色素变性（RP），是世界范围内致盲的主要原因。人类不能再生视网膜，然而，蝾螈（Ambystoma mexicanum），一种实验室培育的蝾螈，可以在整个成年期再生视网膜组织。包括成纤维细胞生长因子（FGF）在内的经典信号通路参与蝾螈的再生。糖胺聚糖（GAG）与FGF的相互作用是该途径中信号转导所必需的。GAG是细胞外基质（ECM）中的阴离子多糖，其已经涉及两栖动物的肢体和透镜再生，然而，尚未在视网膜再生的背景下研究GAG。使用液相色谱质谱法表征天然和脱细胞的蝾螈和猪视网膜的GAG组合物。猪被用作没有视网膜再生能力的哺乳动物脊椎动物模型。硫酸软骨素（CS）是主要的视网膜GAG，其次是硫酸乙酰肝素（HS），透明质酸，硫酸角质素在天然和脱细胞的蝾螈和猪视网膜。与猪视网膜相比，蝾螈视网膜表现出独特的GAG组成模式，包括更高含量的透明质酸。在CS中，在蝾螈视网膜中观察到更高水平的4-和6-O-硫酸化。HS组成是更大的去细胞组织在蝾螈和猪视网膜分别为7.1%和15.4%，并检测到不同的硫酸化模式在蝾螈。我们的研究结果表明，一个独特的GAG组合物配置文件的蝾螈视网膜设置的GAG的作用的基础，在稳态和再生条件的蝾螈视网膜，并可能进一步我们的理解视网膜再生模型。
Retinal degenerative diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are major causes of blindness worldwide. Humans cannot regenerate retina, however, axolotl (Ambystoma mexicanum), a laboratory-bred salamander, can regenerate retinal tissue throughout adulthood. Classic signaling pathways, including fibroblast growth factor (FGF), are involved in axolotl regeneration. Glycosaminoglycan (GAG) interaction with FGF is required for signal transduction in this pathway. GAGs are anionic polysaccharides in extracellular matrix (ECM) that have been implicated in limb and lens regeneration of amphibians, however, GAGs have not been investigated in the context of retinal regeneration. GAG composition is characterized native and decellularized axolotl and porcine retina using liquid chromatography mass spectrometry. Pig was used as a mammalian vertebrate model without the ability to regenerate retina. Chondroitin sulfate (CS) was the main retinal GAG, followed by heparan sulfate (HS), hyaluronic acid, and keratan sulfate in both native and decellularized axolotl and porcine retina. Axolotl retina exhibited a distinctive GAG composition pattern in comparison with porcine retina, including a higher content of hyaluronic acid. In CS, higher levels of 4- and 6-O-sulfation were observed in axolotl retina. The HS composition was greater in decellularized tissues in both axolotl and porcine retinaby 7.1% and 15.4%, respectively, and different sulfation patterns were detected in axolotl. Our findings suggest a distinctive GAG composition profile of the axolotl retina set foundation for role of GAGs in homeostatic and regenerative conditions of the axolotl retina and may further our understanding of retinal regenerative models.
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DOI：
10.4049/jimmunol.0903596
发表时间：
2010-11-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kelly U;Yu L;Kumar P;Ding JD;Jiang H;Hageman GS;Arshavsky VY;Frank MM;Hauser MA;Rickman CB
通讯作者：
Rickman CB
影响因子：
5.3
作者：
Ichijo, H;Kawabata, I
通讯作者：
Kawabata, I
影响因子：
2.9
作者：
Inoue, Yoko;Yoneda, Masahiko;Zako, Masahiro
通讯作者：
Zako, Masahiro
影响因子：
4.4
作者：
Keenan, Tiarnan D. L.;Pickford, Claire E.;Bishop, Paul N.
通讯作者：
Bishop, Paul N.
影响因子：
3.7
作者：
KULYK, WM;ZALIK, SE;DIMITROV, E
通讯作者：
DIMITROV, E