Rates of Mutations and Transcript Errors in the Foodborne Pathogen Salmonella enterica subsp. enterica.
Rates of Mutations and Transcript Errors in the Foodborne Pathogen Salmonella enterica subsp. enterica.
复制标题
食源性病原体肠沙门氏菌亚种的突变率和转录错误率。
DOI：
10.1093/molbev/msac081
复制
发表时间：
2022-04-10
影响因子：
10.7
通讯作者：
Long, Hongan
中科院分区：
文献类型：
--
作者：
Pan, Jiao;Li, Weiyi;Ni, Jiahao;Wu, Kun;Konigsberg, Iain;Rivera, Caitlyn E.;Tincher, Clayton;Gregory, Colin;Zhou, Xia;Doak, Thomas G.;Lee, Heewook;Wang, Yan;Gao, Xiang;Lynch, Michael;Long, Hongan
摘要由于DNA水平的错误是病原体进化的动力，系统地了解突变的速率和分子谱可以指导感染性疾病的避免和治疗。因此，我们在来自不同地理位置、时间分布和遗传背景的18个菌株的768个反复检查的谱系中积累了数万个自发突变。经过136万多代的研究，得到的数据显示每代每个基因组的平均突变率为0.0005，具有显著的种内变异。这是所报道的最低细菌突变率之一，直接支持了该病原体中由高DNA错配修复效率和复制机制保真度引起的高基因组稳定性。致病性基因不表现出加速突变率，因此，突变率升高可能不是毒素和分泌系统多样化的主要决定因素。有趣的是，在转录水平上没有观察到低错误率，这表明复制和转录机制的独特保真度。这项研究促使人们更多地关注甚至是最著名的人类病原体的最基本的进化过程，并加深了对其基因组进化的理解。
Abstract Because errors at the DNA level power pathogen evolution, a systematic understanding of the rate and molecular spectra of mutations could guide the avoidance and treatment of infectious diseases. We thus accumulated tens of thousands of spontaneous mutations in 768 repeatedly bottlenecked lineages of 18 strains from various geographical sites, temporal spread, and genetic backgrounds. Entailing over ∼1.36 million generations, the resultant data yield an average mutation rate of ∼0.0005 per genome per generation, with a significant within-species variation. This is one of the lowest bacterial mutation rates reported, giving direct support for a high genome stability in this pathogen resulting from high DNA-mismatch-repair efficiency and replication-machinery fidelity. Pathogenicity genes do not exhibit an accelerated mutation rate, and thus, elevated mutation rates may not be the major determinant for the diversification of toxin and secretion systems. Intriguingly, a low error rate at the transcript level is not observed, suggesting distinct fidelity of the replication and transcription machinery. This study urges more attention on the most basic evolutionary processes of even the best-known human pathogens and deepens the understanding of their genome evolution.
登录
查看更多内容
影响因子：
30.8
作者：
Holt, Kathryn E.;Parkhill, Julian;Mazzoni, Camila J.;Roumagnac, Philippe;Weill, Francois-Xavier;Goodhead, Ian;Rance, Richard;Baker, Stephen;Maskell, Duncan J.;Wain, John;Dolecek, Christiane;Achtman, Mark;Dougan, Gordon
通讯作者：
Dougan, Gordon
影响因子：
4.5
作者：
Hershberg R;Petrov DA
通讯作者：
Petrov DA
DOI：
10.1073/pnas.2004077118
发表时间：
2021-01-05
影响因子：
11.1
作者：
Fritsch, C.;Gout, J-F;Vermulst, M.
通讯作者：
Vermulst, M.
影响因子：
3.4
作者：
Bobay LM;Ochman H
通讯作者：
Ochman H
DOI：
10.1080/09553005914550241
发表时间：
1959-01-01
期刊：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE
影响因子：
--
作者：
BATEMAN, AJ
通讯作者：
BATEMAN, AJ