Spatial subcellular organelle networks in single cells.
Spatial subcellular organelle networks in single cells.
复制标题
单细胞中的空间亚细胞器网络。
DOI：
10.1038/s41598-023-32474-y
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发表时间：
2023-04-01
影响因子：
4.6
通讯作者：
Coskun, Ahmet F.
中科院分区：
文献类型：
--
作者：
Venkatesan, Mythreye;Zhang, Nicholas;Marteau, Benoit;Yajima, Yukina;Garcia, Nerea Ortiz De Zarate;Fang, Zhou;Hu, Thomas;Cai, Shuangyi;Ford, Adam;Olszewski, Harrison;Borst, Andrew;Coskun, Ahmet F.
细胞器在人类健康和疾病中起着重要作用，如维持体内平衡，调节生长和衰老以及产生能量。细胞中细胞器的多样性不仅存在于细胞类型之间，而且存在于单个细胞之间。因此，在单细胞水平上研究细胞器的分布对于理解细胞功能非常重要。间充质干细胞是多能细胞，已被探索作为治疗多种疾病的治疗方法。研究这些细胞中细胞器的结构可以回答有关其特征和潜力的问题。在此，快速多重免疫荧光（RapMIF）进行了解10个细胞器蛋白质的空间组织和它们之间的相互作用在骨髓（BM）和脐带（UC）间充质干细胞（MSC）。空间相关性，共定位，聚类，统计检验，纹理和形态学分析进行了单细胞水平，揭示了细胞器之间的相互关系和比较的两个MSC亚型。这样的分析工具集表明，与BM MSC相比，UC MSC表现出更高的细胞器表达和伴随有几种其他细胞器的线粒体的空间扩散分布。这种由快速亚细胞蛋白质组成像提供的数据驱动的单细胞方法能够实现个性化的干细胞治疗。
Organelles play important roles in human health and disease, such as maintaining homeostasis, regulating growth and aging, and generating energy. Organelle diversity in cells not only exists between cell types but also between individual cells. Therefore, studying the distribution of organelles at the single-cell level is important to understand cellular function. Mesenchymal stem cells are multipotent cells that have been explored as a therapeutic method for treating a variety of diseases. Studying how organelles are structured in these cells can answer questions about their characteristics and potential. Herein, rapid multiplexed immunofluorescence (RapMIF) was performed to understand the spatial organization of 10 organelle proteins and the interactions between them in the bone marrow (BM) and umbilical cord (UC) mesenchymal stem cells (MSCs). Spatial correlations, colocalization, clustering, statistical tests, texture, and morphological analyses were conducted at the single cell level, shedding light onto the interrelations between the organelles and comparisons of the two MSC subtypes. Such analytics toolsets indicated that UC MSCs exhibited higher organelle expression and spatially spread distribution of mitochondria accompanied by several other organelles compared to BM MSCs. This data-driven single-cell approach provided by rapid subcellular proteomic imaging enables personalized stem cell therapeutics.
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影响因子：
64.5
作者：
Goltsev Y;Samusik N;Kennedy-Darling J;Bhate S;Hale M;Vazquez G;Black S;Nolan GP
通讯作者：
Nolan GP
骨髓、脂肪组织、脐带和胎盘来源的间充质干细胞促血管生成特征的异质性
影响因子：
7.5
作者：
Du WJ;Chi Y;Yang ZX;Li ZJ;Cui JJ;Song BQ;Li X;Yang SG;Han ZB;Han ZC
通讯作者：
Han ZC
影响因子：
48
作者：
Giesen, Charlotte;Wang, Hao A. O.;Bodenmiller, Bernd
通讯作者：
Bodenmiller, Bernd
影响因子：
13.6
作者：
Keren, Leeat;Bosse, Marc;Angelo, Michael
通讯作者：
Angelo, Michael
影响因子：
4.6
作者：
Cappi, Giulia;Dupouy, Diego Gabriel;Ciftlik, Ata Tuna
通讯作者：
Ciftlik, Ata Tuna