Maintenance of proximal and distal cell functions in SV40‐transformed tubular cell lines derived from rabbit kidney cortex
Maintenance of proximal and distal cell functions in SV40‐transformed tubular cell lines derived from rabbit kidney cortex
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兔肾皮质来源的 SV40 转化管状细胞系近端和远端细胞功能的维持
DOI：
10.1002/jcp.1041410128
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发表时间：
1989
影响因子：
5.6
通讯作者：
Pierre M. Ronco
中科院分区：
文献类型：
--
作者：
A. Vandewalle;B. Lelongt;M. Géniteau;B. Baudouin;M. Antoine;S. Estrade;F. Châtelet;P. Verroust;R. Cassingena;Pierre M. Ronco
本文报告的制备和描述的特性，三个肾小管细胞系来源于SV 40感染的原代培养的兔肾皮质细胞，在近端细胞富集。RC.SV1最初来源于在胎牛血清存在下生长的培养物，其表现出低程度的近端分化。随后使细胞适应于在无血清的溶血成分确定的培养基中生长，并显示出近端小管细胞的基本特性，包括发育良好的顶端微绒毛、刷状缘水解酶的强表达、Na+偶联葡萄糖摄取以及暴露于PTH时增加的环AMP产生。其他两种细胞系来源于无血清的动物成分确定培养基中的培养物，并在相同培养基中繁殖。它们的特征在于一些共同的性质，包括罕见和短的微绒毛，顶端水解酶的低表达，以及低或不可检测的Na+依赖性葡萄糖摄取，但不同的是它们通过增加cAMP对各种激素刺激的反应能力。RC.SV2细胞对降钙素敏感，对异丙肾上腺素和PTH敏感程度较低，表明它们可能起源于亨利氏袢的粗升支和远端小管的明亮部分。RC.SV3基本上对异丙肾上腺素和精氨酸加压素有反应，表明起源更远（晚期远端和初始集合小管）。显示近端特征的培养物中远端细胞系的出现可能与远端细胞过度生长有关，如生长动力学分析和与RC.SV1相比RC.SV2中Na+/H+交换活性增加所示。
This paper reports the preparation and describes the properties of three renal tubular cell lines derived using SV40 infection of primary cultures of rabbit kidney cortical cells, enriched in proximal cells. RC.SV1 was initially derived from cultures grown in the presence of fetal calf serum exhibiting a low degree of proximal differentiation. The cells were subsequently adapted to grow in serum‐free hor‐monally defined medium and display basic properties of proximal tubule cells including well‐developed apical microvilli, strong expression of brush‐border hydrolases, Na+‐coupled glucose uptake, and increased cyclic AMP production when exposed to PTH. The other two cell lines were derived from cultures in serum‐free hormonally defined medium and propagated in the same medium. They are characterized by some common properties including rare and short microvilli, low expression of apical hydrolases, and low or undetectable Na+‐dependent glucose uptake, but differ by their abilities to respond by an increase in cAMP to various hormonal stimuli. RC.SV2 cells are sensitive to calcitonin and to a lesser extent to isoproterenol and PTH, suggesting that they may originate from the thick ascending limb of Henle's loop and the bright portion of the distal tubule. RC.SV3 responds essentially to isoproterenol and arginine va‐sopressin, suggesting a more distal origin (late distal and initial collecting tubule). Emergence of distal cell lines from cultures exhibiting proximal characteristics may be related to distal cell overgrowth as suggested by analysis of growth kinetics and increased Na+/H+ exchanger activity in RC.SV2 compared with RC.SV1.
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DOI：
10.1152/ajprenal.1986.251.2.f188
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
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作者：
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DOI：
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发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
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Rabito,CA
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DOI：
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发表时间：
1985
期刊：
The American journal of physiology
影响因子：
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DOI：
10.1152/ajpcell.1987.253.3.c433
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
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作者：
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