Small extracellular vesicles derived from mesenchymal stem cell facilitate functional recovery in spinal cord injury by activating neural stem cells via the ERK1/2 pathway.
Small extracellular vesicles derived from mesenchymal stem cell facilitate functional recovery in spinal cord injury by activating neural stem cells via the ERK1/2 pathway.
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DOI：
10.3389/fncel.2022.954597
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发表时间：
2022
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
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作者：
Spinal cord injury (SCI) causes severe neurological dysfunction leading to a devastating disease of the central nervous system that is associated with high rates of disability and mortality. Small extracellular vesicles (sEVs) derived from human umbilical cord mesenchymal stem cells (hucMSC-sEVs) have been explored as a promising strategy for treating SCI. In this study, we investigated the therapeutic effects of the intralesional administration of hucMSC-sEVs after SCI and determined the potential mechanisms of successful repair by hucMSC-sEVs. In vivo, we established the rat model of SCI. The Basso, Beattie, Bresnahan (BBB) scores showed that hucMSC-sEVs dramatically promoted the recovery of spinal cord function. The results of the hematoxylin–eosin (HE) staining, Enzyme-Linked Immunosorbent Assay (ELISA), and immunohistochemistry showed that hucMSC-sEVs inhibited inflammation and the activation of glia, and promoted neurogenesis. Furthermore, we studied the effect of hucMSC-sEVs on neural stem cells(NSCs) in vitro. We found that hucMSC-sEVs did not improve the migration ability of NSCs, but promoted NSCs to proliferate and differentiate via the ERK1/2 signaling pathway. Collectively, these findings suggested that hucMSC-sEVs promoted the functional recovery of SCI by activating neural stem cells via the ERK1/2 pathway and may provide a new perspective and therapeutic strategy for the clinical application of hucMSC-sEVs in SCI treatment.
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影响因子：
2.4
作者：
Lin, Guan-lin;Wang, Huan;Fang, Huang
通讯作者：
Fang, Huang
DOI：
10.1007/978-1-59745-019-5_18
发表时间：
2010-01-01
期刊：
MOUSE CELL CULTURE: METHODS AND PROTOCOLS
影响因子：
--
作者：
Ahlenius, Henrik;Kokaia, Zaal
通讯作者：
Kokaia, Zaal
影响因子：
16
作者：
Woo, Chang Hee;Kim, Hark Kyun;Cho, Yong Woo
通讯作者：
Cho, Yong Woo
DOI：
10.1016/j.apmr.2017.06.012
发表时间：
2018-03-01
影响因子：
4.3
作者：
Rivers, Carly S.;Fallah, Nader;Noreau, Luc
通讯作者：
Noreau, Luc
影响因子：
56.9
作者：
Clarke, DL;Johansson, CB;Frisén, J
通讯作者：
Frisén, J