Distinct responses of splenic dendritic cell subsets to infection with Listeria monocytogenes: maturation phenotype, level of infection, and T cell priming capacity ex vivo.
Distinct responses of splenic dendritic cell subsets to infection with Listeria monocytogenes: maturation phenotype, level of infection, and T cell priming capacity ex vivo.
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DOI：
10.1016/j.cellimm.2011.03.001
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发表时间：
2011
影响因子：
4.3
通讯作者：
Hiltbold, E. M.
中科院分区：
文献类型：
--
作者：
Mitchell, L. M.;Brzoza-Lewis, K. L.;Henry, C. J.;Grayson, J. M.;Westcott, M. M.;Hiltbold, E. M.
为了确定 DC 亚群在单核细胞增多性李斯特菌保护性免疫发展中的相对贡献，我们检查了感染 Lm 后四个已明确表征的脾 DC 亚群的成熟、细菌负荷和 T 细胞启动能力之间的关系。比较静脉注射后 24、48 和 72 小时的 CD8α+、CD4+ 和 CD8α−CD4− DC 和 B220+ 浆细胞样 DC (pDC) 的丰度和共刺激分子表达。感染。我们进一步确定了与每个 DC 子集相关的细菌负荷及其在 24 hpi 时启动 CD8+ T 细胞的相对能力。 CD8α+ DC 显示出最高水平的成熟度、与活细菌的关联以及 T 细胞活化潜力。其次，CD4+ DC 也成熟了，但与较少的细菌相关，并刺激 T 细胞增殖，但不产生 IFN-γ。 CD8α−CD4− DC 显示出适度的成熟反应，并与大量细菌相关，但未能诱导 T 细胞离体增殖。 pDC表现出强烈的成熟反应，但与可检测到的细菌无关，也未能刺激T细胞活化。最后，我们测量了这些亚群中的细胞因子反应，并确定 IL-12 主要由 CD8+ DC 产生，这与该亚群 DC 诱导 T 细胞中 IFN-γ 产生的能力相关。我们得出的结论是，脾脏中李斯特菌特异性 CD8+ T 细胞的激活最有效地通过感染诱导 CD8α+ DC 亚群的成熟来实现。
To determine the relative contributions of DC subsets in the development of protective immunity to Listeria monocytogenes we examined the relationship between maturation, bacterial burden, and T cell priming capacity of four well characterized subsets of splenic DC following infection with Lm. CD8α+, CD4+, and CD8α−CD4− DC and the B220+ plasmacytoid DC (pDC) were compared for abundance and costimulatory molecule expression at 24, 48, and 72h post i.v. infection. We further determined the bacterial burden associated with each DC subset and their relative capacities to prime CD8+ T cells at 24hpi. The CD8α+ DC displayed the highest level of maturation, association with live bacteria, and T cell activation potential. Second, the CD4+ DC were also mature, yet were associated with fewer bacteria, and stimulated T cell proliferation, but not IFN-γ production. The CD8α−CD4− DC showed a modest maturation response and were associated with a high number of bacteria, but failed to induce T cell proliferation ex vivo. pDC displayed a strong maturation response, but were not associated with detectable bacteria and also failed to stimulate T cell activation. Finally, we measured the cytokine responses in these subsets and determined that IL-12 was produced predominantly by the CD8+ DC, correlating with the ability of this subset DC to induce IFN-γ production in T cells. We conclude that Listeria-specific CD8+ T cell activation in the spleen is most effectively achieved by infection-induced maturation of the CD8α+ DC subset.
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影响因子：
32.4
作者：
Jung, S;Unutmaz, D;Lang, RA
通讯作者：
Lang, RA
影响因子：
4.4
作者：
Farrand, Kathryn J.;Dickgreber, Nina;Hermans, Ian F.
通讯作者：
Hermans, Ian F.
影响因子：
3.1
作者：
Bahjat, Keith S.;Liu, Weiqun;Brockstedt, Dirk G.
通讯作者：
Brockstedt, Dirk G.
影响因子：
32.4
作者：
Aoshi, Taiki;Zinselmeyer, Bernd H.;Miller, Mark J.
通讯作者：
Miller, Mark J.
影响因子：
4.4
作者：
Belz, GT;Shortman, K;Heath, WR
通讯作者：
Heath, WR