Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice.
Oral administration of monogalactosyl diacylglycerol from spinach inhibits colon tumor growth in mice.
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口服菠菜中的单半乳糖二酰基甘油可抑制小鼠结肠肿瘤的生长。
DOI：
10.3892/etm.2012.792
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发表时间：
2013-01
影响因子：
2.7
通讯作者：
Mizushina Y
中科院分区：
文献类型：
--
作者：
Maeda N;Kokai Y;Hada T;Yoshida H;Mizushina Y
此前，我们观察到菠菜中的一种主要糖脂--单半乳糖二酰甘油选择性地抑制哺乳动物复制基因聚合酶α，δ和ε的活性。然而，MGDG在摄入后的功能还不是很清楚。在本研究中，菠菜MGDG在体外对小鼠结肠癌细胞的增殖有抑制作用，其半数致死浓度为24μg/ml。制备环糊精(γ)-环糊精(CD)-MGDG复合体，灌胃给药26天。观察到20 mg/kg当量(Eq.)与赋形剂治疗的对照组相比，CD-MGDG复合体的肿瘤体积减少了60%∼。免疫组织化学分析显示，CD-MGDG复合物组与对照组相比，肿瘤细胞增殖细胞核抗原阳性细胞数减少，有丝分裂减少。此外，CD-MGDG复合体还能显著增加末端脱氧核苷酸转移酶dUTP缺口末端标记(TUTP)阳性的凋亡细胞数，抑制CD31阳性肿瘤血管的生长。这些结果表明，MGDG具有预防癌症和促进健康的潜力。
Previously, we observed that purified monogalactosyl diacylglycerol (MGDG), a major glycoglycerolipid from spinach, selectively inhibits the activities of mammalian replicative DNA polymerases (α, δ and ε). However, the function of MGDG following ingestion is not well-known. In the present study, spinach MGDG suppressed the proliferation of Colon26 mouse colon cancer cells with an LD50 of 24 μg/ml in vitro. γ-cyclodextrin (CD)-MGDG complex was prepared and administered orally following Colon26 mouse tumor adhesion for 26 days. It was observed that 20 mg/kg equivalent (eq.) of the CD-MGDG complex reduced tumor volume by ∼60% compared with that of the vehicle-treated controls. In immunohistochemical analysis, the CD-MGDG complex group showed a decreased number of proliferating cell nuclear antigen (PCNA)-positive cells and reduction of mitosis in the tumor cells compared with the control group. In addition, the CD-MGDG complex increased the number of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive apoptotic cells and inhibited CD31-positive tumor blood vessel growth significantly. These results suggest that MGDG has the potential for cancer prevention and health promotion.
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影响因子：
5.6
作者：
Kuriyama, I;Musumi, K;Mizushina, Y
通讯作者：
Mizushina, Y
影响因子：
3.8
作者：
ROUGHAN, PG;BATT, RD
通讯作者：
BATT, RD
影响因子：
5.6
作者：
Sugawara, T;Miyazawa, T
通讯作者：
Miyazawa, T
DOI：
10.1111/j.1349-7006.2000.tb00887.x
发表时间：
2000-10-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
Hanashima, S;Mizushina, Y;Sakaguchi, K
通讯作者：
Sakaguchi, K
DOI：
10.1016/j.bbrc.2006.11.021
发表时间：
2007-01-12
影响因子：
3.1
作者：
Maeda, Naoki;Kokai, Yasuo;Mizushina, Yoshiyuki
通讯作者：
Mizushina, Yoshiyuki