3-dimensional imaging modalities for phenotyping genetically engineered mice.
3-dimensional imaging modalities for phenotyping genetically engineered mice.
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DOI：
10.1177/0300985811429814
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发表时间：
2012-01
影响因子：
2.4
通讯作者：
Wilson D
中科院分区：
文献类型：
--
作者：
Powell KA;Wilson D
多种三维（3D）数字成像模式可用于基因工程小鼠的全身评估：磁共振显微镜（MRM），X射线显微计算机断层扫描（microCT），光学投影断层扫描（OPT），episcopic和冷冻成像，以及超声生物显微镜（UBM）。胚胎和成年小鼠的表型可以完成在显微镜或近显微镜空间分辨率使用这些方式。MRM和microCT特别适合于在器官水平评估结构信息，而episcopic和OPT成像在细胞水平提供来自分子荧光成像的结构和功能信息。UBM可用于监测子宫内胚胎发育的纵向变化。标本在制备过程中不会发生显著变化，并且可以在其天然方向上观察结构。随着这一令人兴奋的领域的发展，快速自动化数据采集和高通量表型分析技术已经开发并不断改进。
A variety of 3-dimensional (3D) digital imaging modalities are available for whole-body assessment of genetically engineered mice: magnetic resonance microscopy (MRM), X-ray microcomputed tomography (microCT), optical projection tomography (OPT), episcopic and cryoimaging, and ultrasound biomicroscopy (UBM). Embryo and adult mouse phenotyping can be accomplished at microscopy or near microscopy spatial resolutions using these modalities. MRM and microCT are particularly well-suited for evaluating structural information at the organ level, whereas episcopic and OPT imaging provide structural and functional information from molecular fluorescence imaging at the cellular level. UBM can be used to monitor embryonic development longitudinally in utero. Specimens are not significantly altered during preparation, and structures can be viewed in their native orientations. Technologies for rapid automated data acquisition and high-throughput phenotyping have been developed and continually improve as this exciting field evolves.
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DOI：
10.1002/ar.a.20308
发表时间：
2006-03-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Bloom, MW;Murakami, S;Duke, PJ
通讯作者：
Duke, PJ
影响因子：
10.8
作者：
Dunlevy, Louisa;Bennett, Mike;Mohun, Timothy
通讯作者：
Mohun, Timothy
影响因子：
3.3
作者：
Berrios-Otero, Cesar A.;Wadghiri, Youssef Zaim;Turnbull, Daniel H.
通讯作者：
Turnbull, Daniel H.
影响因子：
2.5
作者：
Aoyagi, Hidekazu;Tsuchikawa, Kohzo;Iwasaki, Shin-ichi
通讯作者：
Iwasaki, Shin-ichi
影响因子：
4.5
作者：
Dullin, Christian;Missbach-Guentner, Jeannine;Vogel, Wolfgang F.;Grabbe, Eckhardt;Alves, Frauke
通讯作者：
Alves, Frauke