Evidence for the occurrence of two sympatric sibling species within the Anopheles (Kerteszia) cruzii complex in southeast Brazil and the detection of asymmetric introgression between them using a multilocus analysis.
Evidence for the occurrence of two sympatric sibling species within the Anopheles (Kerteszia) cruzii complex in southeast Brazil and the detection of asymmetric introgression between them using a multilocus analysis.
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DOI：
10.1186/1471-2148-13-207
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发表时间：
2013-09-24
影响因子：
3.4
通讯作者：
Peixoto AA
中科院分区：
文献类型：
--
作者：
Rona LD;Carvalho-Pinto CJ;Peixoto AA
克氏按蚊（双翅目：蚊科）是巴西南部和东南部人类和猿猴疟疾寄生虫的主要媒介。早期使用染色体倒位、同工酶和许多分子标记的研究表明，An。 cruzii 是一个物种复合体。在这项研究中，采用了六个基因座、三个生物钟基因和三个编码核糖体蛋白的多基因座方法，以更详细地研究按蚊之间的遗传分化。来自弗洛里亚诺波利斯-圣卡塔琳娜州（巴西南部）和伊塔蒂亚亚-里约热内卢州（巴西东南部）的克鲁兹种群。首先进行分析比较弗洛里亚诺波利斯和 Itatiaia，然后比较 Itatiaia 的两个假定的同域早期物种（Itatiaia A 和 Itatiaia B）。分析显示弗洛里亚诺波利斯和 Itatiaia（一起考虑 Itatiaia A 和 Itatiaia B）之间以及同域 Itatiaia A 和 Itatiaia B 之间的 FST 值较高，无论其功能如何。此外，使用 IM 程序，使用所有位点一起在 Florianópolis 和 Itatiaia 之间（考虑 Itatiaia A 和 B）没有发现强烈的迁徙迹象，但在 Itatiaia A 和 Itatiaia B 之间，结果显示仅向 Itatiaia B 方向有迁徙的证据。多位点分析的结果表明 Florianópolis 和 Itatiaia 代表 An 的不同物种。 cruzii 复合体的分歧约为 0.6 Mya，并且 Itatiiaa 样本由两个同域的初始物种 A 和 B 组成，其分歧约为 0.2 Mya。尽管某些位点存在很大差异，但后两个物种之间发现了不对称基因渗入。
Anopheles (Kerteszia) cruzii (Diptera: Culicidae) is a primary vector of human and simian malaria parasites in southern and southeastern Brazil. Earlier studies using chromosome inversions, isoenzymes and a number of molecular markers have suggested that An. cruzii is a species complex. In this study, a multilocus approach using six loci, three circadian clock genes and three encoding ribosomal proteins, was carried out to investigate in more detail the genetic differentiation between the An. cruzii populations from Florianópolis–Santa Catarina (southern Brazil) and Itatiaia–Rio de Janeiro States (southeastern Brazil). The analyses were performed first comparing Florianópolis and Itatiaia, and then comparing the two putative sympatric incipient species from Itatiaia (Itatiaia A and Itatiaia B). The analysis revealed high FST values between Florianópolis and Itatiaia (considering Itatiaia A and B together) and also between the sympatric Itatiaia A and Itatiaia B, irrespective of their function. Also, using the IM program, no strong indication of migration was found between Florianópolis and Itatiaia (considering Itatiaia A and B together) using all loci together, but between Itatiaia A and Itatiaia B, the results show evidence of migration only in the direction of Itatiaia B. The results of the multilocus analysis indicate that Florianópolis and Itatiaia represent different species of the An. cruzii complex that diverged around 0.6 Mya, and also that the Itatiaia sample is composed of two sympatric incipient species A and B, which diverged around 0.2 Mya. Asymmetric introgression was found between the latter two species despite strong divergence in some loci.
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影响因子：
3.8
作者：
Donnelly, MJ;Pinto, J;Lehmann, T
通讯作者：
Lehmann, T
影响因子：
2.8
作者：
Forattini, OP;Kakitani, I;de Brito, M
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de Brito, M
影响因子：
4.9
作者：
Grazziotin, Felipe G.;Monzel, Markus;Bonatto, Sandro L.
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Bonatto, Sandro L.
影响因子：
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作者：
HAFFER, J
通讯作者：
HAFFER, J
影响因子：
3.3
作者：
Hey, Jody;Pinho, Catarina
通讯作者：
Pinho, Catarina