Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells.
Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells.
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DOI：
10.1038/s41598-018-27203-9
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发表时间：
2018-06-20
影响因子：
4.6
通讯作者：
Carrara S
中科院分区：
文献类型：
--
作者：
Kilic T;Valinhas ATS;Wall I;Renaud P;Carrara S
包括外泌体（50 ~ 150nm的膜颗粒）在内的纳米细胞外囊泡（ev）由于携带亲本细胞的遗传信息而成为很有前景的癌症生物标志物。然而，这些囊泡的灵敏检测仍然是一个挑战。在这里，我们提出了一种无标记电化学传感器来测量低氧和常氧MCF-7细胞的ev分泌水平。传感器设计包括两个连续的步骤；i)金电极表面功能化用于抗cd81抗体和ii) ev捕获。采用差分脉冲伏安法（DPV）和电化学阻抗谱法（EIS）对电动汽车进行无标签检测。传感器的工作线性范围为102 ~ 109 ev /ml， EIS和DPV检测的LOD分别为77 ev /ml和379 ev /ml。选择性试验采用血富集蛋白RhD。采用酶联免疫吸附法（ELISA）和纳米颗粒跟踪分析法（NTA）对人乳腺癌MCF-7细胞株的ev分泌水平进行了比较，以评价该生物传感器的性能。设计的无标记电化学传感器用于定量cocl2诱导的缺氧对ev分泌的增强，与常压条件相比增加了1.23倍。
Nanoscale extracellular vesicles (EVs) including exosomes (50–150 nm membrane particles) have emerged as promising cancer biomarkers due to the carried genetic information about the parental cells. However the sensitive detection of these vesicles remains a challenge. Here we present a label-free electrochemical sensor to measure the EVs secretion levels of hypoxic and normoxic MCF-7 cells. The sensor design includes two consecutive steps; i) Au electrode surface functionalization for anti-CD81 Antibody and ii) EVs capture. The label-free detection of EVs was done via Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS). The working linear range for the sensor was 102–109 EVs/ml with an LOD 77 EVs/mL and 379 EVs/ml for EIS and DPV based detection. A blood-abundant protein, RhD was used for the selectivity test. In order to assess the performance of the biosensor, the level of EVs secretion by the human breast cancer MCF-7 cell line was compared with enzyme-linked immunosorbent assays (ELISA) and Nanoparticle Tracking Analysis (NTA). Designed label-free electrochemical sensors utilized for quantification of EVs secretion enhancement due to CoCl2-induced hypoxia and 1.23 fold increase with respect to normoxic conditions was found.
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DOI：
10.1002/advs.201600522
发表时间：
2017-05
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
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作者：
Shin SR;Kilic T;Zhang YS;Avci H;Hu N;Kim D;Branco C;Aleman J;Massa S;Silvestri A;Kang J;Desalvo A;Hussaini MA;Chae SK;Polini A;Bhise N;Hussain MA;Lee H;Dokmeci MR;Khademhosseini A
通讯作者：
Khademhosseini A
外泌体：乳腺癌的新兴生物标志物
影响因子：
--
作者：
Jia Y;Chen Y;Wang Q;Jayasinghe U;Luo X;Wei Q;Wang J;Xiong H;Chen C;Xu B;Hu W;Wang L;Zhao W;Zhou J
通讯作者：
Zhou J
影响因子：
7.4
作者：
Rupert, Deborah L. M.;Lasser, Cecilia;Hook, Fredrik
通讯作者：
Hook, Fredrik
新型纳米传感技术用于外泌体检测和分析。
影响因子：
6.1
作者：
Im H;Lee K;Weissleder R;Lee H;Castro CM
通讯作者：
Castro CM
影响因子：
16
作者：
Bobrie A;Colombo M;Krumeich S;Raposo G;Théry C
通讯作者：
Théry C