Integrin adhesions: who's on first? What's on second? Connections between FAK and talin.
Integrin adhesions: who's on first? What's on second? Connections between FAK and talin.
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DOI：
10.4161/cam.20488
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发表时间：
2012-07
影响因子：
3.2
通讯作者：
Schlaepfer DD
中科院分区：
文献类型：
--
作者：
Lawson C;Schlaepfer DD
细胞迁移需要黏附位点组装和周转的协调。新生粘附形成的典型模型假设整合素与细胞外基质（ECM）蛋白结合导致细胞骨架蛋白（如talin和paxillin）快速募集到整合素细胞质结构域。整合素-talin簇被认为招募和激活酪氨酸激酶，如局灶黏附激酶（FAK）。然而，这种连锁的分子联系仍未得到解决。我们最近的研究结果支持另一种模型，即FAK将talin招募到小鼠胚胎成纤维细胞和人卵巢癌细胞中β1整合素介导的粘附的新位点。这依赖于FAK和talin之间的直接结合相互作用，独立于talin与β1整合素的直接结合。在此，我们讨论了新生和成熟黏附之间的差异，FAK、talin和paxillin之间的相互作用，FAK激活的可能机制，以及FAK-talin复合物如何通过增加黏附周转来促进细胞运动。
Cell migration requires the coordination of adhesion site assembly and turnover. Canonical models for nascent adhesion formation postulate that integrin binding to extracellular matrix (ECM) proteins results in the rapid recruitment of cytoskeletal proteins such as talin and paxillin to integrin cytoplasmic domains. It is thought that integrin-talin clusters recruit and activate tyrosine kinases such as focal adhesion kinase (FAK). However, the molecular connections of this linkage remain unresolved. Our recent findings support an alternative model whereby FAK recruits talin to new sites of β1 integrin-mediated adhesion in mouse embryonic fibroblasts and human ovarian carcinoma cells. This is dependent on a direct binding interaction between FAK and talin and occurs independently of direct talin binding to β1 integrin. Herein, we discuss differences between nascent and mature adhesions, interactions between FAK, talin and paxillin, possible mechanisms of FAK activation and how this FAK-talin complex may function to promote cell motility through increased adhesion turnover.
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DOI：
10.1038/nrm2957
发表时间：
2010-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
16
作者：
Lim, Ssang-Taek;Chen, Xiao Lei;Llic, Dusko
通讯作者：
Llic, Dusko
影响因子：
3.4
作者：
Choi, Colin K.;Zareno, Jessica;Horwitz, Alan Rick
通讯作者：
Horwitz, Alan Rick
影响因子：
4
作者：
Schaller, Michael D.
通讯作者：
Schaller, Michael D.
影响因子：
9.2
作者：
Serrels, Bryan;Sandilands, Emma;Frame, Margaret C.
通讯作者：
Frame, Margaret C.