Unique Epigenetic Programming Distinguishes Regenerative Spermatogonial Stem Cells in the Developing Mouse Testis.
Unique Epigenetic Programming Distinguishes Regenerative Spermatogonial Stem Cells in the Developing Mouse Testis.
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DOI：
10.1016/j.isci.2020.101596
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发表时间：
2020-10-23
期刊：
影响因子：
5.8
通讯作者：
McCarrey JR
中科院分区：
文献类型：
--
作者：
Cheng K;Chen IC;Cheng CE;Mutoji K;Hale BJ;Hermann BP;Geyer CB;Oatley JM;McCarrey JR
在哺乳动物睾丸中，精原干细胞（ssc）既能自我更新，又能产生启动生精分化的祖细胞。关于南南合作国家和前身国家在多大程度上在功能上不同的问题仍然存在。在这里，我们提供了第一个哺乳动物生殖细胞表观基因组的多参数综合分析，可与ENCODE项目对bb100体细胞类型所做的分析相媲美。区分SSC富集和祖细胞富集的精原细胞的差异表达基因表现出不同的组蛋白修饰模式，特别是H3K27ac和H3K27me3。基序分析预测了可能调节精原细胞亚型特异性命运的转录因子，免疫组织化学和基因特异性染色质免疫沉淀分析证实了这些因子子集的靶基因结合的亚型特异性差异。综上所述，这些结果表明，ssc和祖细胞表现出不同的表观遗传特征，这些精原细胞亚型要么作为ssc自我更新并维持再生能力，要么作为祖细胞失去再生能力并启动谱系承诺。精原干细胞自我更新并产生祖精原细胞差异基因表达反映干细胞与分化细胞的命运差异基因表达显示差异表观遗传编程基序分析预测细胞类型特异性表观遗传编程的调控因子发育遗传学组学
Spermatogonial stem cells (SSCs) both self-renew and give rise to progenitors that initiate spermatogenic differentiation in the mammalian testis. Questions remain regarding the extent to which the SSC and progenitor states are functionally distinct. Here we provide the first multiparametric integrative analysis of mammalian germ cell epigenomes comparable with that done for >100 somatic cell types by the ENCODE Project. Differentially expressed genes distinguishing SSC- and progenitor-enriched spermatogonia showed distinct histone modification patterns, particularly for H3K27ac and H3K27me3. Motif analysis predicted transcription factors that may regulate spermatogonial subtype-specific fate, and immunohistochemistry and gene-specific chromatin immunoprecipitation analyses confirmed subtype-specific differences in target gene binding of a subset of these factors. Taken together, these results show that SSCs and progenitors display distinct epigenetic profiling consistent with these spermatogonial subtypes being differentially programmed to either self-renew and maintain regenerative capacity as SSCs or lose regenerative capacity and initiate lineage commitment as progenitors. Spermatogonial stem cells both self-renew and give rise to progenitor spermatogonia Differential gene expression reflects stem versus differentiating cell fates Differentially expressed genes show differential epigenetic programming Motif analysis predicts regulators of cell type-specific epigenetic programming Developmental Genetics; Omics
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影响因子：
64.8
作者：
Hajkova, Petra;Ancelin, Katia;Waldmann, Tanja;Lacoste, Nicolas;Lange, Ulrike C.;Cesari, Francesca;Lee, Caroline;Almouzni, Genevieve;Schneider, Robert;Surani, M. Azim
通讯作者：
Surani, M. Azim
影响因子：
64.8
作者：
Ernst, Jason;Kheradpour, Pouya;Mikkelsen, Tarjei S.;Shoresh, Noam;Ward, Lucas D.;Epstein, Charles B.;Zhang, Xiaolan;Wang, Li;Issner, Robbyn;Coyne, Michael;Ku, Manching;Durham, Timothy;Kellis, Manolis;Bernstein, Bradley E.
通讯作者：
Bernstein, Bradley E.
影响因子：
16
作者：
Calo, Eliezer;Wysocka, Joanna
通讯作者：
Wysocka, Joanna
H3K9ME3依赖性异染色质：细胞命运变化的屏障。
DOI：
10.1016/j.tig.2015.11.001
发表时间：
2016-01
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Becker JS;Nicetto D;Zaret KS
通讯作者：
Zaret KS
影响因子：
44.1
作者：
Guo J;Grow EJ;Mlcochova H;Maher GJ;Lindskog C;Nie X;Guo Y;Takei Y;Yun J;Cai L;Kim R;Carrell DT;Goriely A;Hotaling JM;Cairns BR
通讯作者：
Cairns BR