3D electron microscopy and volume-based bouton sorting reveal the selectivity of inputs onto geniculate relay cell and interneuron dendrite segments.
3D electron microscopy and volume-based bouton sorting reveal the selectivity of inputs onto geniculate relay cell and interneuron dendrite segments.
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DOI：
10.3389/fnana.2023.1150747
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发表时间：
2023
影响因子：
2.9
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中科院分区：
文献类型：
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视网膜神经节细胞诱发的视觉信号在被发送到皮层之前，受到各种突触输入的修改和调节，这些突触输入冲击外侧膝状核细胞。膝状输入在膝状细胞类型的离散树突段上聚集或形成微电路的选择性可能为膝状电路的网络特性和通过视觉平行通路的差分信号处理提供了结构基础。在我们的研究中，我们旨在揭示小鼠外侧膝状核中形态学上可识别的中继细胞类型和中间神经元的输入选择性模式。我们使用两组扫描块面电子显微镜（SBEM）图像堆栈和重建软件手动重建终端按钮和树突段。首先，使用无偏终端抽样（UTS）方法和统计建模，我们确定了基于体积的膝状钮扣分类标准，以确定其假定的起源。膝状末端钮扣根据先前描述的线粒体形态分为视网膜和非视网膜类别，可以根据其钮扣体积分布进一步分为多个亚群。基于形态学标准的非视网膜终端由五个不同的亚群组成，包括小尺寸的皮质丘脑和胆碱能钮扣，两个中等尺寸的假定gaba能输入，以及一个包含深色线粒体的大尺寸钮扣类型。视网膜终末也由四个不同的亚群组成。然后将这些亚群的切断标准应用于中继细胞或中间神经元重建树突段上突触的终端数据集。使用网络分析方法，我们发现视网膜和皮质末端在假定的x型细胞树突片段上几乎完全分离，其特征是葡萄状附属物和三联体。在这些细胞上，中间神经元附属物与视网膜和其他中等大小的终末混合在肾小球内形成三联体。相比之下，另一种假定的y型细胞显示树突附着点，并接受所有终端类型，但没有突触位置的选择性；他们没有参与三合会。此外，X型、Y型和中间神经元树突接收到的视网膜突触和皮质突触的贡献是不同的，因此，中间神经元树突的输入有60%以上来自视网膜，而X型和Y型细胞的输入分别为20%和7%。这些结果揭示了不同起源的膝状细胞类型的突触输入的网络特性的差异。
The visual signals evoked at the retinal ganglion cells are modified and modulated by various synaptic inputs that impinge on lateral geniculate nucleus cells before they are sent to the cortex. The selectivity of geniculate inputs for clustering or forming microcircuits on discrete dendritic segments of geniculate cell types may provide the structural basis for network properties of the geniculate circuitry and differential signal processing through the parallel pathways of vision. In our study, we aimed to reveal the patterns of input selectivity on morphologically discernable relay cell types and interneurons in the mouse lateral geniculate nucleus. We used two sets of Scanning Blockface Electron Microscopy (SBEM) image stacks and Reconstruct software to manually reconstruct of terminal boutons and dendrite segments. First, using an unbiased terminal sampling (UTS) approach and statistical modeling, we identified the criteria for volume-based sorting of geniculate boutons into their putative origins. Geniculate terminal boutons that were sorted in retinal and non-retinal categories based on previously described mitochondrial morphology, could further be sorted into multiple subpopulations based on their bouton volume distributions. Terminals deemed non-retinal based on the morphological criteria consisted of five distinct subpopulations, including small-sized putative corticothalamic and cholinergic boutons, two medium-sized putative GABAergic inputs, and a large-sized bouton type that contains dark mitochondria. Retinal terminals also consisted of four distinct subpopulations. The cutoff criteria for these subpopulations were then applied to datasets of terminals that synapse on reconstructed dendrite segments of relay cells or interneurons. Using a network analysis approach, we found an almost complete segregation of retinal and cortical terminals on putative X-type cell dendrite segments characterized by grape-like appendages and triads. On these cells, interneuron appendages intermingle with retinal and other medium size terminals to form triads within glomeruli. In contrast, a second, presumed Y-type cell displayed dendrodendritic puncta adherentia and received all terminal types without a selectivity for synapse location; these were not engaged in triads. Furthermore, the contribution of retinal and cortical synapses received by X-, Y- and interneuron dendrites differed such that over 60% of inputs to interneuron dendrites were from the retina, as opposed to 20% and 7% to X- and Y-type cells, respectively. The results underlie differences in network properties of synaptic inputs from distinct origins on geniculate cell types.
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DOI：
10.1002/cne.22223
发表时间：
2010-03-01
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Bickford ME;Slusarczyk A;Dilger EK;Krahe TE;Kucuk C;Guido W
通讯作者：
Guido W
影响因子：
3.3
作者：
Cavdar, Safiye;Hacioglu, Husniye;Onat, Filiz
通讯作者：
Onat, Filiz
DOI：
10.1111/ejn.13917
发表时间：
2019-04
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Bickford ME
通讯作者：
Bickford ME
影响因子：
2.5
作者：
Dankowski, A;Bickford, ME
通讯作者：
Bickford, ME
DOI：
10.1007/bf00339284
发表时间：
1964-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
COLONNIER, M;GUILLERY, RW
通讯作者：
GUILLERY, RW