Persistent production of colony‐stimulating factor (CSF‐1) by cloned bone marrow stromal cell line D2XRII after X‐irradiation
Persistent production of colony‐stimulating factor (CSF‐1) by cloned bone marrow stromal cell line D2XRII after X‐irradiation
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X 射线照射后克隆骨髓基质细胞系 D2XRII 持续产生集落刺激因子 (CSF-1)
DOI：
10.1002/jcp.1041260311
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发表时间：
1986
影响因子：
5.6
通讯作者：
J. Greenberger
中科院分区：
文献类型：
--
作者：
E. Naparstek;T. Donnelly;R. Shadduck;A. Waheed;K. Wagner;K. Kase;J. Greenberger
The adherent stromal layer in long‐term bone marrow cultures (LTBMC) provides the cellular environment necessary for the in vitro proliferation and differentiation of pluripotential hematopoietic stem cells. The role of humoral hematopoietic growth factors, colony‐stimulating factors (CSF) in the regulation of hematopoietic cell production in this system is poorly understood. We have recently isolated and cloned an adherent cell line, D2XRII, derived from murine LTBMC. Plateau phase 25 cm2 cultures of 2 × 106 D2XRII cells in 8.0 ml produced CSF‐1 (M‐CSF) at around 100–150 units/0.1 ml medium. Following X‐irradiation there was a dose‐dependent decrease in the production of CSF‐1 to a plateau of 50% of control levels at 10,000 rad. Higher doses did not produce a further decrease. The X‐ray dose reducing CSF‐1 production to 50% was 100‐fold above the lethal dose as measured by clonagenic survival following trypsinization and replating. Trypsinized replated viable adherent but nondividing X‐irradiated D2XRII cells were maintained for up to 8 weeks after irradiation and demonstrated continuous production of CSF‐1. The data indicate significant divergence of two biologic effects of X‐irradiation on plateau‐phase marrow stromal cells: physiologic function of adherence and CSF‐1 production, versus proliferative integrity. This divergence of effects may be very relevant to understanding the mechanism of X‐irradiation‐associated marrow suppression and leukemogenesis.
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DOI：
10.1073/pnas.78.11.6963
发表时间：
1981
影响因子：
11.1
作者：
Harigaya,K;Cronkite,EP;Miller,ME;Shadduck,RK
通讯作者：
Shadduck,RK
小鼠长期骨髓培养物体外 X 射线照射对粒细胞-巨噬细胞集落刺激因子产生的生物学影响。
影响因子：
2.6
作者：
Naparstek,E;Donnelly,T;Kase,K;Greenberger,JS
通讯作者：
Greenberger,JS
源自红色和黄色骨髓的兔基质成纤维细胞的表征。
DOI：
10.1084/jem.163.2.400
发表时间：
1986
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bainton,DF;Maloney,MA;Patt,HM;Stern,R
通讯作者：
Stern,R
影响因子：
2.6
作者：
M. Lichtman
通讯作者：
M. Lichtman
从长期骨髓培养物中建立的淋巴因子依赖性细胞系上存在白细胞介素 3 特异性受体的证据。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Palaszynski,EW;Ihle,JN
通讯作者：
Ihle,JN