A transcriptomics approach uncovers novel roles for poly(ADP-ribosyl)ation in the basal defense response in Arabidopsis thaliana.
A transcriptomics approach uncovers novel roles for poly(ADP-ribosyl)ation in the basal defense response in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0190268
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Bent AF
中科院分区：
文献类型：
--
作者：
Briggs AG;Adams-Phillips LC;Keppler BD;Zebell SG;Arend KC;Apfelbaum AA;Smith JA;Bent AF
多聚ADP-核糖聚合酶(PARP)的药理抑制或拟南芥PARG1(多(ADP-核糖)糖水解酶)的丢失破坏了植物防御的一部分。在本研究中，我们研究了改变的聚(ADP-核糖基)对微生物相关分子模式(MAMPs)、鞭毛蛋白(Flg22)和EF-Tu(Elf18)诱导的早期基因表达的影响。严格的统计分析和筛选确定了178个基因具有MAMP诱导的mRNA丰度模式，这些基因被PARP抑制剂3-氨基苯甲酰胺(3AB)或PARG1基因敲除改变。从已鉴定的178个基因中，选择了50多个拟南芥T-DNA插入系，并对其进行了基础防御反应的改变筛选。在AT3g55630(一种细胞质叶基多谷氨酸合成酶)、At5g15660(包含一个F-box结构域)、At1g47370(Toll-IL受体结构域)和At5g64060(一种预测的果胶甲基酯酶抑制剂)的敲除中，观察到了响应这些MAMPs而在愈伤组织沉积和/或幼苗生长方面的细微变化。基因表达研究中被过度描述的GO术语包括elf18/parg1的“先天免疫反应”，突出了elf18激活的防御相关基因的一个子集，这些基因在parg1植物中的表达发生了变化。这项研究还允许对野生型拟南芥早期对flg22和elf18的mRNA丰度反应进行严格控制的比较，这揭示了许多差异。PARP抑制剂3-甲氧基苯甲酰胺(3MB)也用于基因表达谱，但该抑制剂具有多效性。这项转录组学研究揭示了进一步剖析MAMP诱导的植物免疫反应的靶点、PARP抑制剂的影响以及多(ADP-核糖)调节植物对MAMP反应的分子机制。
Pharmacological inhibition of poly(ADP-ribose) polymerase (PARP) or loss of Arabidopsis thaliana PARG1 (poly(ADP-ribose) glycohydrolase) disrupt a subset of plant defenses. In the present study we examined the impact of altered poly(ADP-ribosyl)ation on early gene expression induced by the microbe-associate molecular patterns (MAMPs) flagellin (flg22) and EF-Tu (elf18). Stringent statistical analyses and filtering identified 178 genes having MAMP-induced mRNA abundance patterns that were altered by either PARP inhibitor 3-aminobenzamide (3AB) or PARG1 knockout. From the identified set of 178 genes, over fifty Arabidopsis T-DNA insertion lines were chosen and screened for altered basal defense responses. Subtle alterations in callose deposition and/or seedling growth in response to those MAMPs were observed in knockouts of At3g55630 (FPGS3, a cytosolic folylpolyglutamate synthetase), At5g15660 (containing an F-box domain), At1g47370 (a TIR-X (Toll-Interleukin Receptor domain)), and At5g64060 (a predicted pectin methylesterase inhibitor). Over-represented GO terms for the gene expression study included "innate immune response" for elf18/parg1, highlighting a subset of elf18-activated defense-associated genes whose expression is altered in parg1 plants. The study also allowed a tightly controlled comparison of early mRNA abundance responses to flg22 and elf18 in wild-type Arabidopsis, which revealed many differences. The PARP inhibitor 3-methoxybenzamide (3MB) was also used in the gene expression profiling, but pleiotropic impacts of this inhibitor were observed. This transcriptomics study revealed targets for further dissection of MAMP-induced plant immune responses, impacts of PARP inhibitors, and the molecular mechanisms by which poly(ADP-ribosyl)ation regulates plant responses to MAMPs.
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影响因子：
4
作者：
Ame, Jean-Christophe;Fouquerel, Elise;Schreiber, Valerie
通讯作者：
Schreiber, Valerie
影响因子：
7.2
作者：
Babiychuk, E;Cottrill, PB;Kushnir, S
通讯作者：
Kushnir, S
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
DOI：
10.1073/pnas.0606526103
发表时间：
2006-11-28
影响因子：
11.1
作者：
Andrabi, Shaida A.;Kim, No Soo;Dawson, Ted M.
通讯作者：
Dawson, Ted M.
影响因子：
2.9
作者：
Albert, Markus;Felix, Georg
通讯作者：
Felix, Georg