Down-regulation of fatty acid binding protein 7 (Fabp7) is a hallmark of the postpartum brain.
Down-regulation of fatty acid binding protein 7 (Fabp7) is a hallmark of the postpartum brain.
复制标题
DOI：
10.1016/j.jchemneu.2018.07.003
复制
发表时间：
2018-10
影响因子：
2.8
通讯作者：
Gammie SC
中科院分区：
文献类型：
--
作者：
Driessen TM;Zhao C;Saenz M;Stevenson SA;Owada Y;Gammie SC
脂肪酸结合蛋白7（Fabp 7）是一种多功能蛋白质，与胶质细胞分化和增殖，神经发生和多种精神健康疾病有关。最近的微阵列研究确定了产后啮齿动物关键脑区Fabp 7表达的强烈下降。鉴于其多样的功能，Fabp 7可能在塑造母体大脑和促进母体表型方面发挥关键作用。本研究旨在研究Fabp 7在产后CNS中的表达谱。实时定量PCR（qPCR）分析表明，Fabp 7 mRNA在产后大脑中持续下调。在所测试的9个产妇护理相关区域中，产后（相对于处女）女性中的7个表现出Fabp 7的显著降低，包括内侧前额叶皮质（mPFC）、外侧隔核（NA）、外侧隔（LS）、终纹背侧床核（BnSTd）、室旁核（PVN）、外侧下丘脑（LH）以及基底外侧和中央杏仁核（BLA/CeA）。对于腹侧被盖区（VTA）和内侧视前区（MPOA）的Fabp 7水平较低的母亲，但水平的变化没有达到显着性。共聚焦显微镜显示Fabp 7蛋白表达在LS β mRNA中的发现。具体而言，尾部LS表现出Fabp 7免疫反应性的显著降低，而内侧LS的降低刚好高于显著性。双荧光免疫标记证实了Fabp 7表达细胞的星形胶质细胞表型。总的来说，这项研究表明产后大脑中Fabp 7表达的广泛和显着减少，表明Fabp 7的下调可能是产后大脑的标志，并有助于母体表型。
Fatty acid binding protein 7 (Fabp7) is a versatile protein that is linked to glial differentiation and proliferation, neurogenesis, and multiple mental health disorders. Recent microarray studies identified a robust decrease in Fabp7 expression in key brain regions of the postpartum rodents. Given its diverse functions, Fabp7 could play a critical role in sculpting the maternal brain and promoting the maternal phenotype. The present study aimed at investigating the expression profile of Fabp7 across the postpartum CNS. Quantitative real-time PCR (qPCR) analysis showed that Fabp7 mRNA was consistently down-regulated across the postpartum brain. Of the 9 maternal care-related regions tested, seven exhibited significant decreases in Fabp7 in postpartum (relative to virgin) females, including medial prefrontal cortex (mPFC), nucleus accumbens (NA), lateral septum (LS), bed nucleus of stria terminalis dorsal (BnSTd), paraventricular nucleus (PVN), lateral hypothalamus (LH), and basolateral and central amygdala (BLA/CeA). For both ventral tegmental area (VTA) and medial preoptic area (MPOA) levels of Fabp7 were lower in mothers, but levels of changes did not reach significance. Confocal microscopy revealed that protein expression of Fabp7 in the LS paralleled mRNA findings. Specifically, the caudal LS exhibited a significant reduction in Fabp7 immunoreactivity, while decreases in medial LS were just above significance. Double fluorescent immunolabeling confirmed the astrocytic phenotype of Fabp7-expressing cells. Collectively, this research demonstrates a broad and marked reduction in Fabp7 expression in the postpartum brain, suggesting that down-regulation of Fabp7 may serve as a hallmark of the postpartum brain and contribute to the maternal phenotype.
登录
查看更多内容
影响因子：
3.7
作者：
Eisinger BE;Zhao C;Driessen TM;Saul MC;Gammie SC
通讯作者：
Gammie SC
影响因子：
2.4
作者：
Driessen TM;Eisinger BE;Zhao C;Stevenson SA;Saul MC;Gammie SC
通讯作者：
Gammie SC
影响因子：
3.3
作者：
HATTON, GI;YANG, QZ;COBBETT, P
通讯作者：
COBBETT, P
影响因子：
2.9
作者：
FLEMING, AS;VACCARINO, F;LUEBKE, C
通讯作者：
LUEBKE, C
影响因子：
4.8
作者：
BRIDGES, RS
通讯作者：
BRIDGES, RS