Dynamics and roles of phragmoplast microfilaments in cell plate formation during cytokinesis of tobacco BY-2 cells
Dynamics and roles of phragmoplast microfilaments in cell plate formation during cytokinesis of tobacco BY-2 cells
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烟草BY-2细胞胞质分裂过程中成膜体微丝在细胞板形成中的动态和作用
DOI：
10.1007/s11434-009-0265-5
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Baluska F
中科院分区：
文献类型：
--
作者：
Zhang Y;Zhang WJ;Menzel D;Ren HY;Baluska F
成膜体是植物细胞分裂过程中形成细胞板的一种特殊器官。已知微丝(MFS)参与了膜质结构的形成，但膜质MFS的动态分布和作用尚不清楚。本研究以稳定表达微丝报告基因GFP-fABD2的烟草BY-2细胞系为材料，观察了膜生体起始和后期侧向扩展过程中MFS的精确结构和动态变化。三维成像显示，膜质MFS是由后期重组子核之间出现的两组MFS迁移到中间带并形成两层微丝阵列而启动的。FM4-染色的小泡在两个MFS群体之间聚集并与细胞板融合。末端重叠的两层膜质微丝阵列总是围绕着离心式扩张的细胞板。低浓度的乳链菌素B部分破坏中期的MFS，抑制细胞板的固结，阻止细胞板侧向扩张，而高浓度的乳链菌素B抑制细胞周期的进展。在膜原生质体启动后对细胞进行处理，导致细胞板的扩张停止。我们的观察为细胞质分裂过程中膜质MFS的精确结构和动力学提供了新的见解，并表明动态的膜质MFS在细胞板的形成中是重要的。
The phragmoplast is a special apparatus that functions in establishing a cell plate in dividing plant cells. It is known that microfilaments (MFs) are involved in constituting phragmoplast structure, but the dynamic distribution and role of phragmoplast MFs are far from being understood. In this study, the precise structure and dynamics of MFs during the initiation and the late lateral expansion of the phragmoplast were observed by using a tobacco BY-2 cell line stably expressing the microfilament reporter construct GFP-fABD2. Three-dimensional imaging showed that the phragmoplast MFs were initiated by two populations of MFs emerging between the reconstituting daughter nuclei at anaphase, which migrated to the mid-zone and gave rise to two layers of microfilament arrays. FM4-64 stained vesicles accumulated and fused with the cell plate between the two populations of MFs. The two layers of microfilament arrays of phragmoplast with ends overlapped always surrounded the centrifugally expanding cell plate. Partial disruption of MFs at metaphase by low concentration of latrunculin B resulted in the inhibition of the cell plate consolidation and the blockage of cell plate lateral expansion, whereas high concentration of latrunculin B restrained the progression of the cell cycle. Treating the cell after the initiation of phragmoplast led to the cease of the expansion of the cell plate. Our observations provide new insights into the precise structure and dynamics of phragmoplast MFs during cytokinesis and suggest that dynamic phragmoplast MFs are important in cell plate formation.
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肌动蛋白微丝变化对植物细胞力学中细胞板发育的贡献的定量分析。
影响因子：
5.3
作者：
Higaki, Takumi;Kutsuna, Natsumaro;Sano, Toshio;Hasezawa, Seiichiro
通讯作者：
Hasezawa, Seiichiro
影响因子：
4
作者：
A. Cleary;B. Gunning;G. Wasteneys;P. K. Hepler
通讯作者：
A. Cleary;B. Gunning;G. Wasteneys;P. K. Hepler
影响因子：
2.9
作者：
Dahong Zhang;P. Wadsworth;P. K. Hepler
通讯作者：
Dahong Zhang;P. Wadsworth;P. K. Hepler
DOI：
10.1105/tpc.9.10.1815
发表时间：
1997-10
期刊：
The Plant cell
影响因子：
--
作者：
A. Valster;E. S. Pierson;R. Valenta;P. K. Hepler;A. Emons
通讯作者：
A. Valster;E. S. Pierson;R. Valenta;P. K. Hepler;A. Emons
DOI：
--
发表时间：
1986
期刊：
--
影响因子：
--
作者：
J. Parke;Christopher C. J. Miller;B. Anderton
通讯作者：
J. Parke;Christopher C. J. Miller;B. Anderton