Design and application of genetically encoded biosensors.

Design and application of genetically encoded biosensors.
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DOI:
10.1016/j.tibtech.2010.12.004
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发表时间:
2011-03
影响因子:
17.3
通讯作者:
McCombs, Janet E.
McCombs, Janet E.
中科院分区:
工程技术1区
文献类型:
--
作者:
Palmer, Amy E.;Qin, Yan;Park, Jungwon Genevieve;McCombs, Janet E.

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在过去的5-10年中,绿色荧光蛋白(GFP)及其许多衍生物的能量已被用于开发基因编码的荧光生物传感器。这些传感器作为质粒DNA掺入细胞或生物体中,其导致细胞的转录和翻译机制表达功能性传感器。迄今为止,已经开发了100多种不同的基因编码生物传感器,用于离子、分子和酶等多种目标。这种传感器有助于为活细胞和整个生物体的实时生物化学提供一个窗口,并为细胞的内部运作提供前所未有的洞察力。
In the past 5–10 years, the power of the green fluorescent protein (GFP) and its numerous derivatives has been harnessed toward the development of genetically encoded fluorescent biosensors. These sensors are incorporated into cells or organisms as plasmid DNA, which leads the transcriptional and translational machinery of the cell to express a functional sensor. To date, over 100 different genetically encoded biosensors have been developed for targets as diverse as ions, molecules and enzymes. Such sensors are instrumental in providing a window into the real-time biochemistry of living cells and whole organisms, and are providing unprecedented insight into the inner workings of a cell.
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