Measurement of ethylene and methane production in a temperate forest soil using inhibition of acetylene and carbon monoxide

Measurement of ethylene and methane production in a temperate forest soil using inhibition of acetylene and carbon monoxide
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利用乙炔和一氧化碳的抑制作用测量温带森林土壤中乙烯和甲烷的产生

DOI:
10.1007/s11434-008-0085-z
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发表时间:
2008-05
期刊:
科学通报(英文版)
影响因子:
--
通讯作者:
Kazuyuki, Inubushi
Kazuyuki, Inubushi
中科院分区:
其他
文献类型:
--
作者:
Xu XingKai;Kazuyuki, Inubushi

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在室内研究了乙炔(C_2H_2)浓度对温带松林土壤中乙烯和甲烷积累和消耗的影响,以及松林中乙烯和甲烷的原位产生和氮源对这两种产生的冲洗效应。此外,在顶部空间中的C2 H2浓度超过50 Pa的C2 H2引起的森林土壤中的乙烯和甲烷的消耗率比那些没有C2 H2减少了95%以上。此外,在50至10,000 Pa C2 H2范围内添加乙炔在森林土壤中诱导了类似的甲烷积累速率。因此,可以得出结论,存在于超过50 Pa的C2 H2在顶部空间是一种有效的方法来测量甲烷产生的森林土壤。当C2 H2浓度大于50 Pa时,顶空乙烯浓度显著增加(P <0.05),表明乙炔在森林土壤中被还原为乙烯。使用0.5 kPa C2 H2的抑制与在短期内抑制乙炔还原的5 kPa一氧化碳组合,观察到松树林土壤中的原位甲烷产生速率(218 ± 26 μg C m− 2 h −1(μg C/m2/h,下同))大于原位乙烯产生速率(92 ± 6 μg C m− 2 h −1)。氮源如尿素、尿素+硝化抑制剂双氰胺和硝酸钾的添加均能使乙烯和甲烷的产生速率比对照增加5倍,尤其是后者(P <0.05)。研究结果可为不同立地森林土壤乙烯和甲烷释放量的现场测定提供参考。
We studied in the laboratory the effects of acetylene (C2H2) concentrations on the accumulation and consumption of ethylene and methane in a temperate pine forest soil, andin situethylene and methane production and flush effects of nitrogen sources on both productions in the pine forest stand (Pinus sylvestrisL.). The addition of C2H2at concentrations more than 50 Pa C2H2in the headspace caused a more than 95% reduction in rates of ethylene and methane consumption in forest soil compared to those with no C2H2. Furthermore, addition of acetylene within a range of 50 to 10, 000 Pa C2H2induced a similar rate of methane accumulation in forest soil. Hence, it can be concluded that presence of more than 50 Pa C2H2in the headspace is an effective method to measure methane production in forest soil. The addition of C2H2at concentrations more than 50 Pa C2H2induced an increasing concentration of ethylene in the headspace (P⩽0.05), indicating the reduction of acetylene to ethylene in forest soil. Using inhibition of 0.5 kPa C2H2in combination with 5 kPa carbon monoxide that inhibits the reduction of acetylene in a short term, it was observed that there was a largerin situmethane production rate (218 ± 26 μg C m−2h−1(μg C per square meter per hour, the same below)) thanin situethylene production rate (92 ± 6 μg C m−2h−1) in the pine forest soil. The addition of nitrogen sources such as urea, urea plus a nitrification inhibitor dicyandiamide, and potassium nitrate, could induce a 5-fold greater increase in rates ofin situethylene and methane production compared to those in the control, particularly in the latter (P⩽0.05). The results can promotein situmeasurement of ethylene and methane production in forest soils at different sites.
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