Real-time monitoring of the pH microenvironment at the interface of multispecies biofilm and dental composites.
Real-time monitoring of the pH microenvironment at the interface of multispecies biofilm and dental composites.
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DOI：
10.1016/j.aca.2022.339589
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发表时间：
2022-04-08
影响因子：
6.2
通讯作者：
Koley, Dipankar
中科院分区：
文献类型：
--
作者：
Nguyen, Anh Tuan;Goswami, Subir;Ferracane, Jack;Koley, Dipankar
细菌介导的局部pH变化在改变树脂牙科复合材料在动态环境（如口腔）中的完整性方面起着重要作用。为了解决这个问题，我们开发了一种直径为300 μ m的柔性固态电位pH微传感器，能够在10天以上的真实的时间内检测和定量多物种生物膜和牙科树脂界面处的局部pH微环境。我们使用氟化聚（3，4-乙撑二氧噻吩）作为我们新开发的pH传感器的背接触，沿着PVC基离子选择性膜和PTFE AF涂层。高时间分辨率pH数据表明，对于树脂复合物或载玻片基底，前2天的pH值分别从7变化到6和7变化到5.8，然后在剩余8天内分别在6.5到6和6到5.5之间波动。我们可以观察到由生物膜内的乳酸产生介导的pH值的波动，以及pH值恢复到7。然而，随着蔗糖循环的连续进料，酸产生开始超过缓冲能力，并将局部pH降低至接近5.5。在生物膜上方没有观察到这种变化或波动，因为pH保持在7.0 ± 0.2持续10天。生物膜内pH的局部实时监测表明，生物膜下方的pH变化可能导致对底层材料及其界面的损害，但无法在生物膜外部感测到。
Bacterial-mediated local pH change plays an important role in altering the integrity of resin dental composite materials in a dynamic environment such as the oral cavity. To address this, we developed a 300-μm-diameter, flexible, solid-state potentiometric pH microsensor capable of detecting and quantifying the local pH microenvironment at the interface of multispecies biofilm and dental resin in real time over 10 days. We used fluorinated poly(3,4-ethylenedioxythiophene) as the back contact in our newly developed pH sensor, along with a PVC-based ion-selective membrane and PTFE-AF coating. The high temporal resolution pH data demonstrated pH changes from 7 to 6 and 7 to 5.8 for the first 2 days and then fluctuated between 6.5 to 6 and 6 to 5.5 for the remaining 8 days with the resin composite or glass slide substrate respectively. We could observe the fluctuations in pH mediated by lactic acid production within the biofilm and the reestablishment of pH back to 7. However, acid production started to overwhelm buffering capacity with the continuous feed of sucrose cycles and reduced the local pH nearer to 5.5. No such changes or fluctuations were observed above the biofilm, as the pH remained at 7.0 ± 0.2 for 10 days. The localized real-time monitoring of the pH within the biofilm showed that the pH shift underneath the biofilm could lead to damage to the underlying material and their interface but cannot be sensed external to the biofilm.
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影响因子：
3.7
作者：
Schlafer S;Raarup MK;Meyer RL;Sutherland DS;Dige I;Nyengaard JR;Nyvad B
通讯作者：
Nyvad B
影响因子：
8.9
作者：
Rajan, Teena S.;Read, Tania L.;Macpherson, Julie, V
通讯作者：
Macpherson, Julie, V
影响因子：
4.4
作者：
Örtengren, U;Andersson, F;Karlsson, S
通讯作者：
Karlsson, S
影响因子：
9.2
作者：
Hollmann B;Perkins M;Chauhan VM;Aylott JW;Hardie KR
通讯作者：
Hardie KR
影响因子：
7.4
作者：
Joshi VS;Sheet PS;Cullin N;Kreth J;Koley D
通讯作者：
Koley D