Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation.
Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation.
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DOI：
10.1038/s41522-021-00221-8
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发表时间：
2021-06-17
影响因子：
9.2
通讯作者：
Hardie KR
中科院分区：
文献类型：
--
作者：
Hollmann B;Perkins M;Chauhan VM;Aylott JW;Hardie KR
了解生物膜动态的环境微生态将使我们能够发现、管理和开发这些群落。生物膜的组成和结构已经得到了深入的研究；然而，人们对目前存在的环境微生物技术知之甚少。这主要是因为缺乏具有所需测量灵敏度和分辨率的工具来检测这些变化。我们描述了比率荧光pH敏感纳米传感器的应用，作为一种工具，实时观察生物膜中生理pH的变化。纳米传感器包括两个pH敏感的荧光团，共价包裹在惰性聚丙烯酰胺纳米颗粒基质中的一个参考的pH不敏感的荧光团中。该纳米传感器被用来分析两种生物被膜模型的实时三维pH变化：(I)条件致病菌铜绿假单胞菌和(Ii)口腔致病菌变形链球菌。通过纳米传感器实时检测糖代谢，为寻找改善口腔健康的治疗方案提供了潜在的应用。
Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components and architecture of biofilms have been interrogated in depth; however, little is known about the environmental microniches present. This is primarily because of the absence of tools with the required measurement sensitivity and resolution to detect these changes. We describe the application of ratiometric fluorescent pH-sensitive nanosensors, as a tool, to observe physiological pH changes in biofilms in real time. Nanosensors comprised two pH-sensitive fluorophores covalently encapsulated with a reference pH-insensitive fluorophore in an inert polyacrylamide nanoparticle matrix. The nanosensors were used to analyse the real-time three-dimensional pH variation for two model biofilm formers: (i) opportunistic pathogen Pseudomonas aeruginosa and (ii) oral pathogen Streptococcus mutans. The detection of sugar metabolism in real time by nanosensors provides a potential application to identify therapeutic solutions to improve oral health.
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影响因子：
9.5
作者：
Fulaz, Stephanie;Hiebner, Dishon;Casey, Eoin
通讯作者：
Casey, Eoin
影响因子：
4.4
作者：
D'Urzo, Nunzia;Martinelli, Manuele;Maione, Domenico
通讯作者：
Maione, Domenico
DOI：
10.1007/5584_2015_5002
发表时间：
2016-01-01
期刊：
ADVANCES IN MICROBIOLOGY, INFECTIOUS DISEASES AND PUBLIC HEALTH, VOL 1
影响因子：
--
作者：
Brackman, Gilles;Coenye, Tom
通讯作者：
Coenye, Tom
影响因子：
4.2
作者：
Aylott, JW
通讯作者：
Aylott, JW
影响因子：
17.1
作者：
Chauhan, Veeren M.;Orsi, Gianni;Aylott, Jonathan W.
通讯作者：
Aylott, Jonathan W.