DNA barcoding for community ecology--how to tackle a hyperdiverse, mostly undescribed Melanesian fauna.
DNA barcoding for community ecology--how to tackle a hyperdiverse, mostly undescribed Melanesian fauna.
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为社区生态学的DNA条形码 - 如何应对Hyperdever，主要是未描述的Melanesian动物群。
DOI：
10.1371/journal.pone.0028832
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Riedel A
中科院分区：
文献类型：
--
作者：
Tänzler R;Sagata K;Surbakti S;Balke M;Riedel A
三翅目象甲广泛分布于美拉尼西亚各地，在新几内亚种类繁多。它们是天然林的主要特征，具有狭窄的海拔地带性。由于“分类学上的障碍”，它们在群落生态学中的应用被排除在外。我们从新几内亚的7个地区采集了6,500个标本；对270个形态种的1,002个标本进行了DNA测序。目的以3%的阈值对一个精化的数据集(排除9个隐种)进行聚类，结果显示324个遗传类群( = 类群数量相对于形态种的数量高估了20.0%的物种多样性，或120.0%的一致性)和85.6%的分类准确率(DNA群与基于形态的物种假说完全一致的比例)。在8%的临界值时，一致性和准确性最好。GMYC分析显示328个实体(21.5%被高估)，227个GMYC实体(分类准确率84.1%)。这两种方法都优于副分类学家(低估了19%；分类准确率为31.6%)。在多个采样区发现的物种数量以东部高地和休恩最高(S相似性指数0.07，4个共有种)；所有地区的⅓没有物种重叠。当物种表现出明显的地理结构时，DNA条形码方法的成功率最低。总体而言，三翅目昆虫在新几内亚表现出高度的α和β多样性。DNA条形码是生物多样性调查的一种极好的工具，但如果包括距离较近的地点，成功率可能会下降。超多样化的三翅目昆虫是评估美拉尼西亚森林残留物的有用分类群，可以进行比迄今为止常用的脊椎动物分类群更精细的分析。我们的协议应该有助于建立其他高度多样化的动物群组，作为群落生态的目标类群。测序提供了关于令人难以置信的多样性的分类群的客观数据，但大多没有坚实的分类学基础，应该有助于为最终正式命名新物种铺平道路。
Trigonopterus weevils are widely distributed throughout Melanesia and hyperdiverse in New Guinea. They are a dominant feature in natural forests, with narrow altitudinal zonation. Their use in community ecology has been precluded by the “taxonomic impediment”. We sampled >6,500 specimens from seven areas across New Guinea; 1,002 specimens assigned to 270 morphospecies were DNA sequenced. Objective clustering of a refined dataset (excluding nine cryptic species) at 3% threshold revealed 324 genetic clusters (DNA group count relative to number of morphospecies = 20.0% overestimation of species diversity, or 120.0% agreement) and 85.6% taxonomic accuracy (the proportion of DNA groups that “perfectly” agree with morphology-based species hypotheses). Agreement and accuracy were best at an 8% threshold. GMYC analysis revealed 328 entities (21.5% overestimation) with 227 perfect GMYC entities (84.1% taxonomic accuracy). Both methods outperform the parataxonomist (19% underestimation; 31.6% taxonomic accuracy). The number of species found in more than one sampling area was highest in the Eastern Highlands and Huon (Sørensen similarity index 0.07, 4 shared species); ⅓ of all areas had no species overlap. Success rates of DNA barcoding methods were lowest when species showed a pronounced geographical structure. In general, Trigonopterus show high α and β-diversity across New Guinea. DNA barcoding is an excellent tool for biodiversity surveys but success rates might drop when closer localities are included. Hyperdiverse Trigonopterus are a useful taxon for evaluating forest remnants in Melanesia, allowing finer-grained analyses than would be possible with vertebrate taxa commonly used to date. Our protocol should help establish other groups of hyperdiverse fauna as target taxa for community ecology. Sequencing delivers objective data on taxa of incredible diversity but mostly without a solid taxonomic foundation and should help pave the road for the eventual formal naming of new species.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
10.7
作者：
Pineau, P;Henry, M;Vartanian, JP
通讯作者：
Vartanian, JP
影响因子：
10.1
作者：
Keesing, F
通讯作者：
Keesing, F
影响因子：
2.2
作者：
Astrin, Jonas J.;Stueben, Peter E.
通讯作者：
Stueben, Peter E.
影响因子：
6.5
作者：
Meier, Rudolf;Shiyang, Kwong;Ng, Peter K. L.
通讯作者：
Ng, Peter K. L.