Detection of skin cancer margins in Mohs excisions with high-speed strip mosaicing confocal microscopy: a feasibility study.
Detection of skin cancer margins in Mohs excisions with high-speed strip mosaicing confocal microscopy: a feasibility study.
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DOI：
10.1111/bjd.12444
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Nehal K
中科院分区：
文献类型：
--
作者：
Larson B;Abeytunge S;Seltzer E;Rajadhyaksha M;Nehal K
荧光共聚焦镶嵌显微镜是一种新兴的技术，可以直接在切除的组织中快速成像核和形态细节，而不需要冰冻或固定切片处理。在Mohs切除中，基底细胞癌(BCC)的诊断具有很高的敏感性和特异性。为了转化为临床试验和可能的常规实施，最近开发了一种新的、更快的方法，称为条状镶嵌共聚焦显微镜。对荧光条镶嵌共聚焦显微镜检测Mohs切除皮肤癌边缘进行初步评估。17例Mohs患者的组织以条状马赛克的形式进行成像。每个马赛克被分成两半(亚马赛克)，并由莫氏外科医生分级，这名医生对病理视而不见。将34例亚马赛克与相应的Mohs病理进行比较。34张亚马赛克图像的分辨率、对比度和拼接质量都很好。正常皮肤的成分，包括表皮、真皮、真皮附件和皮下组织，很容易被显示出来。初步检测皮肤癌切缘的灵敏度和特异度为94%。这种新的条状镶嵌方法代表了共聚焦显微镜在大面积切除组织成像方面的又一进步。在Mohs手术中，条状镶嵌可以快速评估新鲜切除的基底细胞癌边缘，并可作为冰冻病理的辅助手段。
Fluorescence confocal mosaicing microscopy is an emerging technology for rapid imaging of nuclear and morphologic detail directly in excised tissue, without the need for frozen or fixed section processing. Basal cell carcinomas (BCCs) can be detected with high sensitivity and specificity in Mohs excisions with this approach. For translation to clinical trials and toward potentially routine implementation, a new and faster approach called strip mosaicing confocal microscopy was recently developed. To perform a preliminary assessment of fluorescence strip mosaicing confocal microscopy for detecting skin cancer margins in Mohs excisions. Tissue from 17 Mohs cases was imaged in the form of strip mosaics. Each mosaic was divided into two halves (submosaics) and graded by a Mohs surgeon, who was blinded to the pathology. The 34 submosaics were compared to the corresponding Mohs pathology. The overall image quality was excellent for resolution, contrast and stitching in the 34 submosaics. Components of normal skin including the epidermis, dermis, dermal appendages and subcutaneous tissue were easily visualized. Preliminary measure of sensitivity and specificity was 94% for detecting skin cancer margins. The new strip mosaicing approach represents another advance in confocal microscopy for imaging of large areas of excised tissue. Strip mosaicing may enable rapid assessment of BCC margins in fresh excisions during Mohs surgery and may serve as an adjunct for frozen pathology.
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DOI：
10.1111/j.1610-0387.2011.07692.x
发表时间：
2011-07-01
影响因子：
3.6
作者：
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作者：
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影响因子：
3.6
作者：
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影响因子：
6.5
作者：
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