Plant Microbiome Identification and Characterization

Plant Microbiome Identification and Characterization
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植物微生物组鉴定和表征

DOI:
10.1002/cppb.20048
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发表时间:
2017
影响因子:
--
通讯作者:
Lebeis, L. Sarah
Lebeis, L. Sarah
中科院分区:
--
文献类型:
--
作者:
Lebeis, L. Sarah

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为了充分利用植物微生物组改变的潜力来改善植物健康,必须使用可靠的方法来制备和表征微生物组样品。培养独立研究的力量在于,它们允许表征新的微生物群落成员,但只有在不同研究小组之间一致代表的微生物成员才有可能成为广泛适用的治疗方法。植物微生物组成员的鉴定可能受到几个实验阶段的影响,包括设计、样品制备、核酸提取、测序和分析。因此,这里描述的协议旨在强调在开始植物微生物组研究之前实验者应该考虑的关键步骤。©2017 by John Wiley & Sons, Inc。
To fully exploit the potential of plant microbiome alterations to improve plant health, reliable methods must be used to prepare and characterize microbiome samples. The power of culture‐independent studies is that they allow the characterization of novel microbial community members, but only microbial members consistently represented between different research groups are likely to become broadly applicable treatments. The identification of plant microbiome members can be affected by several experimental stages, including design, sample preparation, nucleic acid extraction, sequencing, and analysis. The protocols described here therefore aim to highlight crucial steps that experimenters should consider before beginning a plant microbiome study. © 2017 by John Wiley & Sons, Inc.
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